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Immunohistochemical analysis of paraffin-embedded human spleen, using Bcl-2 Antibody.
Product group Antibodies
Boster Bio
Anti-Bcl-2 Rabbit Monoclonal AntibodyM00040-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetBCL2
Western blot analysis of Bcl-2 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate (M00040-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2 monoclonal antibody (Catalog # M00040-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL2
Product group Antibodies
References
Boster Bio
Anti-Bcl-2 Rabbit Monoclonal AntibodyM00040-2
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetBCL2
Western blot analysis of Rac1/2/3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate.
Product group Antibodies
Boster Bio
Anti-Rac1/2/3 Rabbit Monoclonal AntibodyM00041
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetRAC1
Western blot analysis of Prothrombin expression in human serum lysate.
Product group Antibodies
Boster Bio
Anti-Prothrombin F2 Monoclonal AntibodyM00044
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetF2
Immunohistochemical analysis of paraffin-embedded human kindey, using VEGF Antibody.
Product group Antibodies
Boster Bio
Anti-VEGF VEGFA Rabbit Monoclonal AntibodyM00045-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetVEGFA
Western blot analysis of P Glycoprotein expression in HeLa cell lysate (M00049). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB1 monoclonal antibody (Catalog # M00049) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCB1
Product group Antibodies
Boster Bio
Anti-P Glycoprotein ABCB1 Rabbit Monoclonal AntibodyM00049
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetABCB1
Western blot analysis of KMT6 / EZH2 expression in HEK293 cell lysate (M00050-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EZH2 monoclonal antibody (Catalog # M00050-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-KMT6 / EZH2 Rabbit Monoclonal AntibodyM00050-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetEZH2
Western blot analysis of KMT6 / EZH2 expression in HeLa cell lysate (M00050). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EZH2 monoclonal antibody (Catalog # M00050) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EZH2
Product group Antibodies
Boster Bio
Anti-KMT6 / EZH2 Rabbit Monoclonal AntibodyM00050
ApplicationsWestern Blot
ReactivityHuman
TargetEZH2
Immunohistochemical analysis of paraffin-embedded human skin, using CD44 Antibody.
Product group Antibodies
Boster Bio
Anti-CD44 Rabbit Monoclonal AntibodyM00052-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetCD44
Western blot analysis of SDF1 expression in Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-SDF1 CXCL12 Rabbit Monoclonal AntibodyM00053
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetCXCL12
Western blot analysis of Fas expression in Ramos cell lysate (M00055). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAS monoclonal antibody (Catalog # M00055) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAS
Product group Antibodies
References
Boster Bio
Anti-Fas/Cd95 Rabbit Monoclonal AntibodyM00055
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetFAS
Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using ER alpha Antibody.
Product group Antibodies
Boster Bio
Anti-ER alpha ESR1 Rabbit Monoclonal AntibodyM00057-2
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetESR1
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