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mmunohistochemical analysis of paraffin-embedded human endometrium, using Sonic Hedgehog Antibody.
Product group Antibodies
Boster Bio
Anti-Sonic Hedgehog SHH Rabbit Monoclonal AntibodyM00058
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetSHH
Immunohistochemical analysis of paraffin-embedded mouse kidney, using Smad3 Antibody(M00059) SMAD3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD3 Antibody (M00059)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
References
Boster Bio
Anti-Smad3 Rabbit Monoclonal AntibodyM00059
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSMAD3
Western blot analysis of Haptoglobin expression in human plasma lysate (M00062). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HP monoclonal antibody (Catalog # M00062) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HP
Product group Antibodies
Boster Bio
Anti-Haptoglobin HP Rabbit Monoclonal AntibodyM00062
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetHP
Immunohistochemical analysis of paraffin-embedded colon cancer, using pan Cadherin Antibody.
Product group Antibodies
References
Boster Bio
Anti-pan Cadherin 1 CDH1 Rabbit Monoclonal AntibodyM00063-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCDH1
IF analysis of COL4A1/E Cadherin using anti-COL4A1/E Cadherin antibody (PB9099/M00063-2) COL4A1/E Cadherin was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-COL4A1 Antibody (PB9099)/mouse anti E Cadherin Antibody(M00063-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) /Cy3 conjugated Goat anti mouse IgG(BA1031),were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Product group Antibodies
References
Boster Bio
Anti-E Cadherin 1 CDH1 Antibody Picoband(r) (monoclonal, 9G2)M00063-2
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCDH1
Immunofluorescent analysis of HeLa cells, using CLOCK Antibody.
Product group Antibodies
Boster Bio
Anti-CLOCK Monoclonal AntibodyM00064
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCLOCK
Immunohistochemical analysis of paraffin-embedded human kidney, using HMGB1 Antibody(M00066-1) HMGB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HMGB1 Antibody (M00066-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
References
Boster Bio
Anti-HMGB1/Hmg 1 Rabbit Monoclonal AntibodyM00066-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHMGB1
Immunofluorescent analysis of BxPC-3 cells, using Insulin Antibody.
Product group Antibodies
References
Boster Bio
Anti-Insulin Rabbit Monoclonal AntibodyM00067-1
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetINS
Western blot analysis of Dystrophin expression in human fetal heart lysate.
Product group Antibodies
Boster Bio
Anti-Dystrophin DMD Rabbit Monoclonal AntibodyM00069
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetDMD
Western blot analysis of beta 2 Adrenergic Receptor expression in A431 cell lysate.
Product group Antibodies
Boster Bio
Anti-beta 2 Adrenergic Receptor ADRB2 Rabbit Monoclonal AntibodyM00072
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetADRB2
Immunohistochemical analysis of paraffin-embedded human brain carcinoma, using FoxO1a Antibody.
Product group Antibodies
References
Boster Bio
Anti-FoxO1a Rabbit Monoclonal AntibodyM00073
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetFOXO1
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Smad4 Antibody(M00074) SMAD4 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD4 Antibody (M00074)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Smad4 Rabbit Monoclonal AntibodyM00074
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSMAD4
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