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Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using B Raf Antibody(M00075) BRAF was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BRAF Antibody (M00075)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-B Raf Rabbit Monoclonal AntibodyM00075
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBRAF
Immunohistochemical analysis of paraffin-embedded human liver carcinoma, using LDL Receptor Antibody
Product group Antibodies
Boster Bio
Anti-LDL Receptor Rabbit Monoclonal AntibodyM00076
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetLDLR
Western blot analysis of Nrf2 expression in HepG2 cell lysate.
Product group Antibodies
Boster Bio
Anti-Nrf2 NFE2L2 Rabbit Monoclonal AntibodyM00078
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetNFE2L2
Immunohistochemical analysis of paraffin-embedded human brain, using Amyloid beta A4 Antibody.
Product group Antibodies
Boster Bio
Anti-Amyloid beta A4 APP Rabbit Monoclonal AntibodyM00081
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAPP
Western blot analysis of Rhodopsin expression in rat eyeball lysate (M00083). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHO monoclonal antibody (Catalog # M00083) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RHO
Product group Antibodies
Boster Bio
Anti-Rhodopsin Rabbit Monoclonal AntibodyM00083
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetRHO
Western blot analysis of PTGS2 expression in A549 cell lysate using anti-PTGS2 antibody (M00084-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGS2 antigen affinity purified polyclonal antibody (Catalog # M00084-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for PTGS2.
Product group Antibodies
Boster Bio
Anti-Cox2 PTGS2 Monoclonal AntibodyM00084-1
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetPTGS2
Western blot analysis of COX2 expression in RAW264.7 cell lysate treated with LPS.
Product group Antibodies
References
Boster Bio
Anti-COX2 PTGS2 Rabbit Monoclonal AntibodyM00084
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetPTGS2
Western blot analysis of RUNX1/RUNX2/RUNX3 expression in MOLT4 cell lysate (M00086). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX1 monoclonal antibody (Catalog # M00086) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX1
Product group Antibodies
Boster Bio
Anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal AntibodyM00086
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRUNX2
Western blot analysis of Rad51 expression in HeLa cell lysate (M00088). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD51 monoclonal antibody (Catalog # M00088) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAD51
Product group Antibodies
Boster Bio
Anti-Rad51 Rabbit Monoclonal AntibodyM00088
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetRAD51
Immunohistochemical analysis of paraffin-embedded rat stomach, using Smad2 Antibody(M00090-1) SMAD2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD2 Antibody (M00090-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Smad2 Rabbit Monoclonal AntibodyM00090-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetSMAD2
Western blot analysis of Smad2 expression in Jurkat cell lysate (M00090). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 monoclonal antibody (Catalog # M00090) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2
Product group Antibodies
Boster Bio
Anti-Smad2 Rabbit Monoclonal AntibodyM00090
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetSMAD2
Western blot analysis of PML expression in 293 cell lysate.
Product group Antibodies
Boster Bio
Anti-PML Rabbit Monoclonal AntibodyM00093
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPML
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