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Western blot analysis of Transferrin expression in Human serum lysate (M00094). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TF monoclonal antibody (Catalog # M00094) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TF
Product group Antibodies
Boster Bio
Anti-Transferrin TF Rabbit Monoclonal AntibodyM00094
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTF
Immunohistochemical analysis of paraffin-embedded human uterus, using PDGF Receptor beta Antibody.
Product group Antibodies
Boster Bio
Anti-PDGF Receptor beta PDGFRB Rabbit Monoclonal AntibodyM00096-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPDGFRB
Western blot analysis of Tau expression in SH-SY5Y cell lysate.
Product group Antibodies
References
Boster Bio
Anti-Tau MAPT Rabbit Monoclonal AntibodyM00097-1
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPT
Western blot analysis of Tau expression in Mouse rat brain lysate (M00097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPT monoclonal antibody (Catalog # M00097) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPT
Product group Antibodies
Boster Bio
Anti-Tau MAPT Rabbit Monoclonal AntibodyM00097
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPT
Western blot analysis of FGFR1 expression in SH-SY5Y cell lysate.
Product group Antibodies
Boster Bio
Anti-FGFR1 Rabbit Monoclonal AntibodyM00098
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetFGFR1
Western blot analysis of KRAS+HRAS+NRAS expression in (1) 293T cell lysate; (2) C6 cell lysate.
Product group Antibodies
Boster Bio
Anti-KRAS+HRAS+NRAS Rabbit Monoclonal AntibodyM00099-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetHRAS
Western blot analysis of Ras expression in (1) C6 cell lysate; (2) Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-Ras NRAS Rabbit Monoclonal AntibodyM00099-2
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetHRAS
Western blot analysis of Ras expression in SH-SY5Y cell lysate.
Product group Antibodies
Boster Bio
Anti-Ras NRAS Rabbit Monoclonal AntibodyM00099
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHRAS
Western blot analysis of ApoB expression in human serum lysate.
Product group Antibodies
Boster Bio
Anti-ApoB/Apolipoprotein B Rabbit Monoclonal AntibodyM00100
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
ReactivityHuman
TargetAPOB
Western blot analysis of extracts from recombinant IL1 beta, using IL1 beta antibody (M00101-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1B monoclonal antibody (Catalog # M00101-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1B
Product group Antibodies
Boster Bio
Anti-IL1 beta Rabbit Monoclonal AntibodyM00101-1
ApplicationsWestern Blot
ReactivityHuman
TargetIL1B
Western blot analysis of IL1 beta expression in recombinant IL1 beta protein (M00101). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1B monoclonal antibody (Catalog # M00101) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1B
Product group Antibodies
Boster Bio
Anti-IL1 beta Rabbit Monoclonal AntibodyM00101
ApplicationsWestern Blot
ReactivityHuman
TargetIL1B
Immunofluorescent analysis of HeLa cells, using ERK1/2 Antibody.
Product group Antibodies
References
Boster Bio
Anti-ERK1/2 MAPK3 Rabbit Monoclonal AntibodyM00104-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK1
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