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Western blot analysis of TIMP2 expression in HeLa cell lysate (M01037-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP2 monoclonal antibody (Catalog # M01037-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMP2
Product group Antibodies
References
Boster Bio
Anti-TIMP2 Rabbit Monoclonal AntibodyM01037-1
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman
TargetTIMP2
Western blot analysis of NeuroD1 expression in Y79 cell lysate (M01038). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEUROD1 monoclonal antibody (Catalog # M01038) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEUROD1
Product group Antibodies
Boster Bio
Anti-NeuroD1 Rabbit Monoclonal AntibodyM01038
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetNEUROD1
Western blot analysis of EDNRB using anti-EDNRB antibody (M01041-1).
Product group Antibodies
Boster Bio
Anti-EDNRB Antibody Picoband(r) (monoclonal, 15C3)M01041-1
ApplicationsWestern Blot
ReactivityHuman
TargetEDNRB
Western blot analysis of Endothelin B Receptor expression in 293T cell lysate.
Product group Antibodies
Boster Bio
Anti-Endothelin B Receptor EDNRB Rabbit Monoclonal AntibodyM01041
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetEDNRB
Western blot analysis of CD79A using anti-CD79A antibody (M01047-1) in human spleen lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD79A antigen affinity purified polyclonal antibody (Catalog # M01047-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-CD79a Monoclonal AntibodyM01047-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCD79A
Western blot analysis of DCX expression in C6 cell lysate (M01053). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCX monoclonal antibody (Catalog # M01053) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DCX
Product group Antibodies
Boster Bio
Anti-DCX/Doublecortin Rabbit Monoclonal AntibodyM01053
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetDCX
Anti-YB1 YBX1 Rabbit Monoclonal Antibody
Product group Antibodies
Boster Bio
Anti-YB1 YBX1 Rabbit Monoclonal AntibodyM01054
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetYBX1
Western blot analysis of Chk1 in (1) K562 cell lysate; (2) PC-12 cell lysate (M01060-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK1 monoclonal antibody (Catalog # M01060-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHEK1
Product group Antibodies
Boster Bio
Anti-Chk1 CHEK1 Rabbit Monoclonal AntibodyM01060-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetCHEK1
Western blot analysis of Chk1 expression in HeLa cell lysate.
Product group Antibodies
Boster Bio
Anti-Chk1 CHEK1 Rabbit Monoclonal AntibodyM01060
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCHEK1
Western blot analysis of MST1 expression in HepG2 cell lysate.
Product group Antibodies
Boster Bio
Anti-MST1 Rabbit Monoclonal AntibodyM01069
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMST1
Immunohistochemical analysis of paraffin-embedded human colon, using alpha smooth muscle Actin Antibody(M01072-1) ACTA2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTA2 Antibody (M01072-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
References
Boster Bio
Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal AntibodyM01072-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetACTA2
Western blot analysis of alpha smooth muscle Actin expression in A431 cell lysate (M01072-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA2 monoclonal antibody (Catalog # M01072-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTA2
Product group Antibodies
Boster Bio
Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal AntibodyM01072-2
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetACTA2
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