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1717 to 1728 of 6.204 products
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Western blot analysis of GBA expression in U87-MG cell lysate.
Product group Antibodies
Boster Bio
Anti-GBA/Glucosylceramidase Rabbit Monoclonal AntibodyM01162
ApplicationsWestern Blot, ImmunoHistoChemistry
Western blot analysis of CHAT expression in SH-SY5Y cell lysate.
Product group Antibodies
Boster Bio
Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal AntibodyM01192
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Immunohistochemical analysis of paraffin-embedded human colon, using ENO1 Antibody(M01250) ENO1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ENO1 Antibody (M01250)overnight at 4°C Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-ENO1/Alpha Enolase Rabbit Monoclonal AntibodyM01250
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Western blot analysis of Glutaminase expression in 293T cell lysate (M01272). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLS monoclonal antibody (Catalog # M01272) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLS
Product group Antibodies
Boster Bio
Anti-Glutaminase GLS Rabbit Monoclonal AntibodyM01272
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of Heparanase 1 expression in K562 cell lysate.
Product group Antibodies
References
Boster Bio
Anti-Heparanase 1 HPSE Monoclonal AntibodyM01313
ApplicationsImmunoPrecipitation, Western Blot
Western blot analysis of p70 S6 Kinase expression in 293T cell lysate (M01475). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KB1 monoclonal antibody (Catalog # M01475) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS6KB1
Product group Antibodies
References
Boster Bio
Anti-p70 S6 Kinase RPS6KB1 Rabbit Monoclonal AntibodyM01475
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Hexokinase 1 Antibody(M01504) HK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HK1 Antibody (M01504)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Hexokinase 1 HK1 Rabbit Monoclonal AntibodyM01504
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of Neutrophil Elastase expression in HL60 cell lysate.
Product group Antibodies
Boster Bio
Anti-Neutrophil Elastase ELANE Rabbit Monoclonal AntibodyM01607
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of LIG4 expression in Ramos cell lysate using anti-LIG4 antibody (M01685). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIG4 antigen affinity purified polyclonal antibody (Catalog # M01685) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for LIG4.
Product group Antibodies
Boster Bio
Anti-DNA Ligase IV Rabbit Monoclonal AntibodyM01685
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of CKB using anti-CKB antibody (M01695) in SHSY5Y cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CKB antigen affinity purified polyclonal antibody (Catalog # M01695) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Creatine kinase B type CKB Monoclonal AntibodyM01695
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Immunohistochemical analysis of paraffin-embedded human colon, using Myosin light chain kinase Antibody(M01697) MYLK was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYLK Antibody (M01697)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
References
Boster Bio
Anti-Myosin light chain kinase MYLK Rabbit Monoclonal AntibodyM01697
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of CES1 expression in U937 cell lysate using anti-CES1 antibody (M01741). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CES1 antigen affinity purified polyclonal antibody (Catalog # M01741) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CES1.
Product group Antibodies
Boster Bio
Anti-Liver Carboxylesterase 1 Monoclonal AntibodyM01741
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
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