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Western blot analysis of PIK3CD using anti-PIK3CD antibody on (1) K562 cell lysate; (2) RAW 264. cell lysate; (3) Rat kidney lysate. (M02269). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3CD antigen affinity purified polyclonal antibody (Catalog # M02269) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-PI 3 Kinase p110 delta PIK3CD Rabbit Monoclonal AntibodyM02269
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Immunohistochemical analysis of paraffin-embedded human kidney, using PI 3 Kinase Class 3 Antibody(M02356) PIK3C3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PIK3C3 Antibody (M02356)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-PI 3 Kinase Class 3 PIK3C3 Rabbit Monoclonal AntibodyM02356
ApplicationsWestern Blot, ImmunoHistoChemistry
Western blot analysis of ATP citrate lyase using anti-ATP citrate lyase antibody (M02372-1).
Product group Antibodies
Boster Bio
Anti-ATP citrate lyase ACLY Antibody Picoband(r) (monoclonal, 5I2)M02372-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Immunohistochemical analysis of paraffin-embedded human brain carcinoma, using ATP citrate lyase Antibody.
Product group Antibodies
References
Boster Bio
Anti-ATP citrate lyase ACLY Rabbit Monoclonal AntibodyM02372
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Immunohistochemical analysis of paraffin-embedded mouse testis, using Caspase-2 Antibody(M02384-1) CASP2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP2 Antibody (M02384-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Caspase-2 CASP2 Rabbit Monoclonal AntibodyM02384-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of Caspase-2 expression in HeLa cell lysate (M02384). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP2 monoclonal antibody (Catalog # M02384) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP2
Product group Antibodies
Boster Bio
Anti-Caspase-2 CASP2 Rabbit Monoclonal AntibodyM02384
ApplicationsWestern Blot
Western blot analysis of RNA Helicase A expression in Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-RNA Helicase A Rabbit Monoclonal AntibodyM02550
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of Caspase-6 p18 expression in Jurkat cell treated with 1uM staurosporine lysate (M02631-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP6 monoclonal antibody (Catalog # M02631-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP6
Product group Antibodies
Boster Bio
Anti-Caspase-6 p18 CASP6 Rabbit Monoclonal AntibodyM02631-1
ApplicationsImmunoPrecipitation, Western Blot
Western blot analysis of Caspase-6 expression in Jurkat cell lysate (M02631-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP6 monoclonal antibody (Catalog # M02631-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP6
Product group Antibodies
Boster Bio
Anti-Caspase-6 CASP6 Rabbit Monoclonal AntibodyM02631-2
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of Caspase-6 expression in MCF-7 cell lysate (M02631). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP6 monoclonal antibody (Catalog # M02631) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP6
Product group Antibodies
Boster Bio
Anti-Caspase-6 CASP6 Rabbit Monoclonal AntibodyM02631
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of Aconitase 1 in (1) mouse kidney lysate; (2) HepG2 cell lysate (M02781). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACO1 monoclonal antibody (Catalog # M02781) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACO1
Product group Antibodies
Boster Bio
Anti-Aconitase 1 ACO1 Rabbit Monoclonal AntibodyM02781
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Western blot analysis of ACSS2 expression in HepG2 cell lysate (M02809). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACSS2 monoclonal antibody (Catalog # M02809) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACSS2
Product group Antibodies
Boster Bio
Anti-ACSS2/Acetyl Coa Synthetase Rabbit Monoclonal AntibodyM02809
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
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