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Western blot analysis of PHD1 in (1) HeLa cell lysate; (2) A549 cell lysate.
Product group Antibodies
Boster Bio
Anti-PHD1/prolyl hydroxylase Rabbit Monoclonal AntibodyM03015
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of Catalase expression in HepG2 cell lysate.
Product group Antibodies
Boster Bio
Anti-Catalase Rabbit Monoclonal AntibodyM03123
ApplicationsWestern Blot, ImmunoHistoChemistry
Immunohistochemical analysis of paraffin-embedded human liver, using Glutamine Synthetase Antibody.
Product group Antibodies
Boster Bio
Anti-Glutamine Synthetase GLUL Rabbit Monoclonal AntibodyM03191
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Western blot analysis of ASNS expression in K562 cell lysate (M03302). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASNS monoclonal antibody (Catalog # M03302) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASNS
Product group Antibodies
Boster Bio
Anti-ASNS/Asparagine Synthetase Rabbit Monoclonal AntibodyM03302
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Immunohistochemical analysis of paraffin-embedded human colon cancer, using PI 3 Kinase p85 beta Antibody(M03363) PIK3R2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PIK3R2 Antibody (M03363)overnight at 4°C Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-PI 3 Kinase p85 beta PIK3R2 Rabbit Monoclonal AntibodyM03363
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of Casein Kinase 2 beta expression in HepG2 cell lysate.
Product group Antibodies
Boster Bio
Anti-Casein Kinase 2 beta Monoclonal AntibodyM03475
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Western blot analysis of Glycogen synthase expression in HeLa cell lysate (M03512). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GYS1 monoclonal antibody (Catalog # M03512) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GYS1
Product group Antibodies
Boster Bio
Anti-Glycogen synthase GYS1 Rabbit Monoclonal AntibodyM03512
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of PDHB using anti-PDHB antibody (M03998) in HeLa cell lysate.. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDHB antigen affinity purified polyclonal antibody (Catalog # M03998) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal AntibodyM03998
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of GOT1 expression in MCF7 cell lysate using anti-GOT1 antibody (M04085). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOT1 antigen affinity purified polyclonal antibody (Catalog # M04085) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for GOT1.
Product group Antibodies
Boster Bio
Anti-Aspartate Aminotransferase Monoclonal AntibodyM04085
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of FoxO4 expression in human heart lysate.
Product group Antibodies
Boster Bio
Anti-FoxO4/Afx Rabbit Monoclonal AntibodyM01819-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetFOXO4
Western blot analysis of FoxO4 expression in 293T cell lysate transfected with FoxO4 (M01819). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO4 monoclonal antibody (Catalog # M01819) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXO4
Product group Antibodies
Boster Bio
Anti-FoxO4/Afx Rabbit Monoclonal AntibodyM01819
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetFOXO4
Western blot analysis of IRF6 expression in Jurkat cell lysate (M01822). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF6 monoclonal antibody (Catalog # M01822) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF6
Product group Antibodies
Boster Bio
Anti-IRF6 Rabbit Monoclonal AntibodyM01822
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetIRF6
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