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Measurement of NAMPT enzymatic activity was performed as described previously [G.C. Elliott, et al.; Anal Biochem 107, 199 (1980)]. The recombinant Nampt was diluted in assay buffer and 10microl per 50microl reaction mix were applied in the reaction mix (20
Product group Proteins / Signaling Molecules
AdipoGen Life Sciences
Protein IDP43490
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Product group Proteins / Signaling Molecules
AdipoGen Life Sciences
Protein IDP20334
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Product group Proteins / Signaling Molecules
AdipoGen Life Sciences
Protein IDP28776
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Product group Proteins / Signaling Molecules
AdipoGen Life Sciences
Protein IDP15090
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Product group Proteins / Signaling Molecules
AdipoGen Life Sciences
Protein IDP22352
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Product group Proteins / Signaling Molecules
AdipoGen Life Sciences
Protein IDP02753
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Pull down assay: a) pull down of hIL-33-His by hST2-Fc b) pull down of hST2-Fc by hIL-33-His 5microg of hST2-Fc (or control Fc protein), 2microg of hIL-33-His (Prod. No. AG-40A-0042) (or control His protein), and protein G resin (or anti-H
Product group Proteins / Signaling Molecules
AdipoGen Life Sciences
Protein IDQ2YEJ5
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Product group Proteins / Signaling Molecules
AdipoGen Life Sciences
Protein IDQ00724
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Product group Proteins / Signaling Molecules
AdipoGen Life Sciences
Protein IDP10909
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Product group Proteins / Signaling Molecules
AdipoGen Life Sciences
Protein IDP04916
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Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and
Product group Proteins / Signaling Molecules
AdipoGen Life Sciences
Protein IDQ2YEJ4
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Product group Proteins / Signaling Molecules
AdipoGen Life Sciences
Protein IDQ549A5
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