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Western blot analysis of CRLF2 using anti-CRLF2 antibody (A04167-1).
Product group Antibodies
Boster Bio
Anti-CRLF2 AntibodyA04167-1
IHC analysis of Hsp105 using anti-Hsp105 antibody (A04168). Hsp105 was detected in paraffin-embedded section of human testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Hsp105 Antibody (A04168) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Hsp105/HSPH1 AntibodyA04168
Western blot analysis of ESM1 using anti-ESM1 antibody (A04169-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human ESM1 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESM1 antigen affinity purified polyclonal antibody (Catalog # A04169-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESM1 at approximately 25KD. The expected band size for ESM1 is at 20KD.
Product group Antibodies
Boster Bio
Anti-ESM1 AntibodyA04169-1
Western blot analysis of TACC1 using anti-TACC1 antibody (A04196).
Product group Antibodies
Boster Bio
Anti-TACC1 Picoband AntibodyA04196
IHC analysis of Peptide YY/PYY using anti-Peptide YY/PYY antibody (A04223-1). Peptide YY/PYY was detected in paraffin-embedded section of human appendicitis tissue tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Peptide YY/PYY Antibody (A04223-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Peptide YY/PYY AntibodyA04223-1
Western blot analysis of MAdCAM1 using anti-MAdCAM1 antibody (A04227-1).
Product group Antibodies
Boster Bio
Anti-MAdCAM1 AntibodyA04227-1
Western blot analysis of CD23 using anti-CD23 antibody (A04237).
Product group Antibodies
Boster Bio
Anti-CD23/FCER2 AntibodyA04237
Western blot analysis of VIP Receptor 1 using anti-VIP Receptor 1 antibody (A04345). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 cell lysates, Lane 2: human PANC-1 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VIP Receptor 1 antigen affinity purified polyclonal antibody (Catalog # A04345) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VIP Receptor 1 at approximately 52KD. The expected band size for VIP Receptor 1 is at 52KD.
Product group Antibodies
Boster Bio
Anti-VIP Receptor 1/VIPR1 AntibodyA04345
Western blot analysis of PLAU using anti-PLAU antibody (A04352-1).
Product group Antibodies
Boster Bio
Anti-PLAU AntibodyA04352-1
Western blot analysis of TNFSF18 using anti-TNFSF18 antibody (A04408-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TNFSF18 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFSF18 antigen affinity purified polyclonal antibody (Catalog # A04408-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFSF18 at approximately 15KD. The expected band size for TNFSF18 is at 15KD.
Product group Antibodies
Boster Bio
Anti-TNFSF18 AntibodyA04408-1
Western blot analysis of IL17E using anti-IL17E antibody (A04429-1).
Product group Antibodies
Boster Bio
Anti-IL17E/IL25 AntibodyA04429-1
Western blot analysis of Noggin using anti-Noggin antibody (A04448-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A375 whole cell lysates, Lane 5: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Noggin antigen affinity purified polyclonal antibody (Catalog # A04448-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Noggin at approximately 26KD. The expected band size for Noggin is at 26KD.
Product group Antibodies
Boster Bio
Anti-Noggin AntibodyA04448-1
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