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Western blot analysis of LRIG1 using anti-LRIG1 antibody (A04613-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRIG1 antigen affinity purified polyclonal antibody (Catalog # A04613-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRIG1 at approximately 145KD. The expected band size for LRIG1 is at 119KD.
Product group Antibodies
Boster Bio
Anti-LRIG1 AntibodyA04613-1
Western blot analysis of FZD3 using anti-FZD3 antibody (A04680-1).
Product group Antibodies
Boster Bio
Anti-FZD3 AntibodyA04680-1
Western blot analysis of GADD45G using anti-GADD45G antibody (A04681-1).
Product group Antibodies
Boster Bio
Anti-GADD45G AntibodyA04681-1
Western blot analysis of HSF2 using anti-HSF2 antibody (A04692).
Product group Antibodies
Boster Bio
Anti-HSF2 AntibodyA04692
Western blot analysis of HOXB1 using anti-HOXB1 antibody (A04724-1).
Product group Antibodies
Boster Bio
Anti-HOXB1 AntibodyA04724-1
Western blot analysis of Tbp using anti-Tbp antibody (A00302).
Product group Antibodies
Boster Bio
Anti-TATA binding protein TBP AntibodyA00302
IHC analysis of VASP using anti-VASP antibody (A00303-1). VASP was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VASP Antibody (A00303-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-VASP Picoband AntibodyA00303-1
Western blot analysis of IFNAR1/Beta R1 using anti-IFNAR1/Beta R1 antibody (A00306-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse stomach tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 70 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNAR1/Beta R1 antigen affinity purified polyclonal antibody (Catalog # A00306-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNAR1/Beta R1 at approximately 64-70KD. The expected band size for IFNAR1/Beta R1 is at 64KD.
Product group Antibodies
Boster Bio
Anti-IFNAR1 Picoband AntibodyA00306-2
Western blot analysis of KCNQ1 using anti-KCNQ1 antibody (A00310-1).
Product group Antibodies
Boster Bio
Anti-KCNQ1 Picoband AntibodyA00310-1
Western blot analysis of CCS using anti-CCS antibody (A00314-1).
Product group Antibodies
Boster Bio
Anti-CCS Picoband AntibodyA00314-1
Western blot analysis of BMP4 using anti-BMP4 antibody (A00321). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human BMP4 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP4 antigen affinity purified polyclonal antibody (Catalog # A00321) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMP4 at approximately 22KD. The expected band size for BMP4 is at 13KD.
Product group Antibodies
Boster Bio
Anti-BMP4 AntibodyA00321
Western blot analysis of BMPR2 using anti-BMPR2 antibody (A00324-1).
Product group Antibodies
Boster Bio
Anti-BMPR2 AntibodyA00324-1
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