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Western blot analysis of CD11a using anti-CD11a antibody (A04466-1).
Product group Antibodies
Boster Bio
Anti-CD11a Picoband AntibodyA04466-1
Western blot analysis of IRF9 using anti-IRF9 antibody (A04485).
Product group Antibodies
Boster Bio
Anti-IRF9 Picoband AntibodyA04485
Western blot analysis of MMP11 using anti-MMP11 antibody (A04490-1).
Product group Antibodies
Boster Bio
Anti-MMP11 Picoband AntibodyA04490-1
Western blot analysis of VEGFB using anti-VEGFB antibody (A04494-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat pancreas tissue lysates, Lane 3: mouse cardiac muscle tissue lysates, Lane 4: mouse skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFB antigen affinity purified polyclonal antibody (Catalog # A04494-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGFB at approximately 29KD. The expected band size for VEGFB is at 22KD.
Product group Antibodies
Boster Bio
Anti-VEGFB AntibodyA04494-1
IHC analysis of VEGFB using anti-VEGFB antibody (A04494-2).
Product group Antibodies
Boster Bio
Anti-VEGFB AntibodyA04494-2
Western blot analysis of VEGFB using anti-VEGFB antibody (A04494-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFB antigen affinity purified polyclonal antibody (Catalog # A04494-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGFB at approximately 29KD. The expected band size for VEGFB is at 22KD.
Product group Antibodies
Boster Bio
Anti-VEGFB AntibodyA04494-3
Western blot analysis of RAB11B using anti-RAB11B antibody (A04526-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat ovary tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse ovary tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB11B antigen affinity purified polyclonal antibody (Catalog # A04526-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB11B at approximately 24KD. The expected band size for RAB11B is at 24KD.
Product group Antibodies
Boster Bio
Anti-RAB11B AntibodyA04526-1
Western blot analysis of SI using anti-SI antibody (A04542-1).
Product group Antibodies
Boster Bio
Anti-SI AntibodyA04542-1
Western blot analysis of COPE using anti-COPE antibody (A04544). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat stomach tissue lysate, Lane 2: rat small intestine tissue lysate, Lane 3: rat pancreas tissue lysate, Lane 4: mouse stomach tissue lysate, Lane 5: mouse small intestine tissue lysate, Lane 6: mouse pancreas tissue lysate, Lane 7: human MCF-7 whole Cell lysatem, Lane 8: human Hela whole Cell lysate, Lane 9: human 22RV1 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COPE antigen affinity purified polyclonal antibody (Catalog # A04544) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COPE at approximately 34KD. The expected band size for COPE is at 34KD.
Product group Antibodies
Boster Bio
Anti-COPE AntibodyA04544
Western blot analysis of MED13 using anti-MED13 antibody (A04545-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED13 antigen affinity purified polyclonal antibody (Catalog # A04545-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED13 at approximately 300KD. The expected band size for MED13 is at 239KD.
Product group Antibodies
Boster Bio
Anti-MED13 AntibodyA04545-1
Western blot analysis of Lymphotactin using anti-Lymphotactin antibody (A04548).
Product group Antibodies
Boster Bio
Anti-Lymphotactin/Xcl1 AntibodyA04548
Flow Cytometry analysis of Jurkat cells using anti-CLEC9A antibody (A04577). Overlay histogram showing Jurkat cells stained with A04577 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CLEC9A Antibody (A04577, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Product group Antibodies
Boster Bio
Anti-CLEC9A AntibodyA04577
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