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Western blot analysis of Phospho-YAP1 (S127) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with FBS+calyculin A.
Product group Antibodies
References
Boster Bio
Anti-Phospho-YAP1 (S127) Rabbit Monoclonal AntibodyP00116
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetYAP1
Western blot analysis of Phospho-Rac1/Cdc42 (Ser71) expression in (1) A431 cell lysate treated with LP; (2) A431 cell lysate (P00119). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC42 monoclonal antibody (Catalog # P00119) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC42
Product group Antibodies
Boster Bio
Anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal AntibodyP00119
ApplicationsWestern Blot
ReactivityHuman
TargetCDC42
Immunohistochemical analysis of paraffin-embedded mouse kidney, using Phospho-eIF4E (S209) antibody.
Product group Antibodies
Boster Bio
Anti-Phospho-eIF4E (S209) Rabbit Monoclonal AntibodyP00135
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetEIF4E
Western blot analysis of Phospho-SHP2 (Y542) expression in (1) NIH/3T3 cell lysates; (2) NIH/3T3 cell lysates treated with PDGF.
Product group Antibodies
Boster Bio
Anti-Phospho-SHP2 (Y542) PTPN11 Rabbit Monoclonal AntibodyP00150
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetPTPN11
Western blot analysis of Phospho-FAK (Y397) expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide (P00151). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTK2 monoclonal antibody (Catalog # P00151) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
References
Boster Bio
Anti-Phospho-FAK (Y397) PTK2 Rabbit Monoclonal AntibodyP00151
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetPTK2
Western blot analysis of Phospho-v-Myb + c-Myb (S11) Antibody expression in Ramos cell lysate (P00157). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYB monoclonal antibody (Catalog # P00157) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYB
Product group Antibodies
Boster Bio
Anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal AntibodyP00157
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetMYB
Western blot analysis of Phospho-IRF3 (S386) expression in MCF7 cell lysate.
Product group Antibodies
Boster Bio
Anti-Phospho-IRF3 (S386) Rabbit Monoclonal AntibodyP00165
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetIRF3
Immunohistochemical analysis of paraffin-embedded rat spleen, using Phospho-Cdk1/2 (T14) Antibody.
Product group Antibodies
Boster Bio
Anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal AntibodyP00209-1
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCDK1
Western blot analysis of Phospho-alpha Synuclein (Ser129) expression in (1) 293T cell lysate; (2) 293T cell lysate transfected with Polo-Like Kinase 2 and alpha Synuclein.
Product group Antibodies
Boster Bio
Anti-Phospho-alpha Synuclein (S129) SNCA Rabbit Monoclonal AntibodyP00215-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetSNCA
Western blot analysis of Synuclein phosphorylation expression in Human fetal brain lysate (P00215-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNCA monoclonal antibody (Catalog # P00215-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNCA
Product group Antibodies
References
Boster Bio
Anti-Phospho-alpha Synuclein (S129) SNCA Rabbit Monoclonal AntibodyP00215-2
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetSNCA
Western blot analysis of Filamin A phosphorylation expression in WT-LRRK2 cell lysate treated LRRK2.
Product group Antibodies
Boster Bio
Anti-Phospho-LRRK2 (S935) Rabbit Monoclonal AntibodyP00221
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetLRRK2
Western blot analysis of Phospho-Vimentin (Ser72) in HeLa cell lysates treated with Calyculin A.
Product group Antibodies
Boster Bio
Anti-Phospho-Vimentin (S72) Rabbit Monoclonal AntibodyP00235
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetVIM
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