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Immunohistochemical analysis of paraffin-embedded human bladder cancer, using Phospho-GATA3 (S308) Antibody.
Product group Antibodies
Boster Bio
Anti-Phospho-GATA3 (S308) Rabbit Monoclonal AntibodyP00593
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetGATA3
Western blot analysis of Phospho-DNA PKcs (Ser2056) expression in alkaline treated Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-Phospho-DNA PKcs (S2056) PRKDC Rabbit Monoclonal AntibodyP00645
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetPRKDC
Western blot analysis of Phospho-Hsp27 (S82) expression in HeLa cell lysate treated with heat shock (P00676-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB1 monoclonal antibody (Catalog # P00676-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPB1
Product group Antibodies
Boster Bio
Anti-Phospho-Hsp27 (S82) HSPB1 Rabbit Monoclonal AntibodyP00676-1
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetHSPB1
Western blot analysis of Phospho-Hsp27 (S78) expression in (1) A431 cell lysate; (2) A431 cell lysate treated with Anisomycin (P00676). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB1 monoclonal antibody (Catalog # P00676) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Phospho-Hsp27 (S78) HSPB1 Rabbit Monoclonal AntibodyP00676
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetHSPB1
Western blot analysis of Phospho-IRE1 (S724) expression in (1) K562 cell lysate treated with AP; (2) Untreated K562 cell lysate.
Product group Antibodies
References
Boster Bio
Anti-Phospho-IRE1 (S724) ERN1 Rabbit Monoclonal AntibodyP00683
ApplicationsWestern Blot
ReactivityHuman
TargetERN1
Western blot analysis of p95/NBS1 phosphorylation expression in Jurkat cell lysate treated with Etopside (P00732). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NBN monoclonal antibody (Catalog # P00732) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NBN
Product group Antibodies
Boster Bio
Anti-Phospho-p95/NBS1 (S343) NBN Rabbit Monoclonal AntibodyP00732
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetNBN
Western blot analysis of Phospho-PKC alpha (T638) expression in (1) Jurkat cell lysate treated with TPA; (2) Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-Phospho-PKC alpha (T638) PRKCA Rabbit Monoclonal AntibodyP00743
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPRKCA
Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-GSK3 beta (Ser9) Antibody(P00791-1) GSK3B was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GSK3B Antibody (P00791-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
References
Boster Bio
Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal AntibodyP00791-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetGSK3B
Western blot analysis of Phospho-ATF2 (T71) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Anisomycin.
Product group Antibodies
Boster Bio
Anti-Phospho-ATF2 (T71) Rabbit Monoclonal AntibodyP00916-3
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetATF2
Western blot analysis of Phospho-AMPK alpha 1 (S496) expression in (1) 293T cell lysate treated with LP; (2) 293T cell lysate (P00994-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAA1 monoclonal antibody (Catalog # P00994-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKAA1
Product group Antibodies
Boster Bio
Anti-Phospho-AMPK alpha 1 (S496) PRKAA1 Rabbit Monoclonal AntibodyP00994-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetPRKAA1
Western blot analysis of Phospho-POLR2A (S2) expression in MCF7 cell lysate treated with Lambda Phosphatase (P01029-1) (1); MCF7 cell lysate (2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2A monoclonal antibody (Catalog # P01029-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR2A
Product group Antibodies
Boster Bio
Anti-Phospho-POLR2A (S2) Rabbit Monoclonal AntibodyP01029-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPOLR2A
Western blot analysis of Phospho-POLR2A (S5) expression in (1) HeLa cell lysate treated with Lambda Phosphatase (P01029-2); (2) HeLa cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2A monoclonal antibody (Catalog # P01029-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Phospho-POLR2A (S5) Rabbit Monoclonal AntibodyP01029-2
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPOLR2A
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