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Western blot analysis of Phospho-BTK (Y223) expression in Raji cell lysate treated with pervanadate (P00245-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BTK monoclonal antibody (Catalog # P00245-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Phospho-BTK (Y223) Rabbit Monoclonal AntibodyP00245-1
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetBTK
Western blot analysis of Phospho-BTK (Y551) expression in Jurkat cell lysate treated with Pervanadate (P00245). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BTK monoclonal antibody (Catalog # P00245) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BTK
Product group Antibodies
Boster Bio
Anti-Phospho-BTK (Y551) Rabbit Monoclonal AntibodyP00245
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetBTK
Western blot analysis of Phospho-HSF1 (S326) expression in HeLa cell lysates treated with heat.
Product group Antibodies
Boster Bio
Anti-Phospho-HSF1 (S326) Rabbit Monoclonal AntibodyP00250
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetHSF1
Western blot analysis of Phospho-FoxO3a (S253) expression in MCF-7 cell lysate treated with IGF.
Product group Antibodies
References
Boster Bio
Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal AntibodyP00252
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetFOXO3
Western blot analysis of Phospho-NF-Kappa B p65 (S529) expression in HeLa cell lysates treated with Calyculin A and TNF-alpha (P00284). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RELA monoclonal antibody (Catalog # P00284) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RELA
Product group Antibodies
Boster Bio
Anti-Phospho-NF-Kappa B p65 (S529) RELA Rabbit Monoclonal AntibodyP00284
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
ReactivityHuman
TargetRELA
Western blot analysis of Phospho-MEK1 (S298) expression in (1) HeLa cell treated with LP lysate; (2) HeLa cell lysate (P00292-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody (Catalog # P00292-3) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K1
Product group Antibodies
Boster Bio
Anti-Phospho-MEK1 (S298) MAP2K1 Rabbit Monoclonal AntibodyP00292-3
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
Western blot analysis of MEK5 expression in (1) HeLa cell lysate; (2) HeLa cell treated with Nocodazole (P00292-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody (Catalog # P00292-4) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K1
Product group Antibodies
Boster Bio
Anti-Phospho-MEK1 (T292) MAP2K1 Rabbit Monoclonal AntibodyP00292-4
ApplicationsWestern Blot
ReactivityHuman
TargetMAP2K1
Western blot analysis of Phospho-Raf1 (S43) expression in (1) HeLa cell lysate treated with LP; (2) HeLa cell lysate (P00446-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAF1 monoclonal antibody (Catalog # P00446-3) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAF1
Product group Antibodies
Boster Bio
Anti-Phospho-Raf1 (S43) Rabbit Monoclonal AntibodyP00446-3
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetRAF1
Western blot analysis of Phospho-Raf1 (S621) expression in (1) HeLa cell lysate treated with LP; (2) HeLa cell lysate (P00446-4).
Product group Antibodies
Boster Bio
Anti-Phospho-Raf1 (S621) Rabbit Monoclonal AntibodyP00446-4
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetRAF1
Western blot analysis of Phospho-Raf1 (S259) expression in (1) 293T cell lysate; (2) 293T cell lysates treated with Okadaic Acid + Calyculin A.
Product group Antibodies
Boster Bio
Anti-Phospho-Raf1 (S259) Rabbit Monoclonal AntibodyP00446-5
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRAF1
Western blot analysis of Phospho-Cyclin E1 (T77) expression in (1) JAR cell treated with Lambda Phosphatase lysate; (2) JAR cell lysate (P00543). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNE1 monoclonal antibody (Catalog # P00543) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNE1
Product group Antibodies
Boster Bio
Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal AntibodyP00543
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCCNE1
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma, using Phospho-Creb (S133) Antibody.
Product group Antibodies
Boster Bio
Anti-Phospho-Creb (S133) CREB1 Rabbit Monoclonal AntibodyP00577-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCREB1
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