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ICC/IF analysis of Tetrahymena themophila cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. The open arrow indicates the micronucleus. Insets are magnified images showing the position of the micronucleus. Both clone 13C2 and 21A10 stained the macronuclear periphery of T. thermophila. 13C2 mAb was highly specific to the macronucleus. In contrast, in addition to clear macronuclear staining, clone 21A10 also stained the micronuclear periphery. This indicates that 21A10 recognizes Nups localizing to the micronucleus such as Nup308 in addition to MacNup98A. Neither could 2H10 nor 414 could stain nuclear periphery of Tetrahymena. Green : Primary antibody Violet : DAPI Dilution : 0.5 microg/ml Fixation : Cold Methanol (-30 degree C) for 30 min
Product group Antibodies
References
GeneTex
NUP98 antibody [13C2] - Comparison ValidatedGTX00693
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Yeast, Other Species
TargetTTHERM_00071070
  • SizePrice
ICC/IF analysis of COS1 cell lysate overexpressed GFP and GST tagged ZIPK fusion protein using GTX00694 GFP antibody [1A5]. Note that fluorescence by the immunofluorescent staining using 1A5 antibody is much stronger than fluorescence of GFP. Red : Primary antibody Green : Fluorescence of GFP
Product group Antibodies
References
GeneTex
GFP antibody [1A5]GTX00694
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityOther Species
  • SizePrice
ICC/IF analysis of Tetrahymena themophila cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. 13C2 mAb was highly specific to the macronucleus. In contrast, in addition to clear macronuclear staining, 21A10 mAb also stained the micronuclear periphery. This indicates that 21A10 mAb recognizes Nups localizing to the micronucleus such as Nup308 in addition to MacNup98A. Neither mABs 2H10 nor 414 could stain nuclear periphery of Tetrahymena. Green : Primary antibody Violet : DAPI Dilution : 0.5 microg/ml Fixation : Cold Methanol (-30 degree C) for 30 min
Product group Antibodies
References
GeneTex
NUP98 antibody [21A10] - Comparison ValidatedGTX00695
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Yeast, Other Species
TargetNUP98
  • SizePrice
ICC/IF analysis of HeLa cells using GTX00696 Nucleoporin p62 antibody [2A11]. Left : Nup62 protein focused on nuclear surface Right : Nup62 protein focused on nuclear rim Dilution : 1:400 Fixation : 4% PFA Permeabilization : 0.5% Triton X-100
Product group Antibodies
References
GeneTex
Nucleoporin p62 antibody [2A11]GTX00696
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Monkey
TargetNUP62
  • SizePrice
ICC/IF analysis of HeLa cells using GTX00697 NUP98 antibody [2H10]. Dilution : 0.5 microg/ml Fixation : Cold Methanol (-30 degree C) for 30 min
Product group Antibodies
References
GeneTex
NUP98 antibody [2H10] - Comparison ValidatedGTX00697
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat, Yeast
TargetNUP98
  • SizePrice
WB analysis of various samples using GTX00698 KPNA4 antibody [3D10]. Lane 1 : HeLa whole cell lysate Lane 2 : COS7 whole cell lysate Lane 3 : L929 whole cell lysate Lane 4 : NRK whole cell lysate Lane 5 : BHK whole cell lysate Lane 6 : MDBK whole cell lysate
Product group Antibodies
References
GeneTex
KPNA4 antibody [3D10]GTX00698
ApplicationsWestern Blot, ELISA
ReactivityBovine, Canine, Hamster, Human, Monkey, Mouse, Primate, Rat
TargetKpna4
  • SizePrice
ICC/IF analysis of C33A cells using GTX00699 SUMO2 + SUMO3 antibody [3H12].
Product group Antibodies
References
GeneTex
SUMO2 + SUMO3 antibody [3H12]GTX00699
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHamster, Human, Mouse, Primate, Rat
TargetSUMO3
  • SizePrice
WB analysis of MDA-MB-468 cell lysate using GTX00700 PHLDA3 antibody [4B6]. PHLDA3 protein was induced by Ad-p53 in MDA-MB-468 cells (left). PHLDA3 expression was inhibited by shRNA targeting PHLDA3 (right).
Product group Antibodies
References
GeneTex
PHLDA3 antibody [4B6] - KO/KD, Orthogonal ValidatedGTX00700
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetPHLDA3
  • SizePrice
ICC/IF analysis of C-33A cells using GTX00701 SUMO1 antibody [4D12]. SUMO-1 colocalizes with SUMO2/3. Left : anti-SUMO-1 antibody (4D12) Middle : anti-SUMO2/3 antibody (3H12) Right : Merged image Dilution : 10 microg/ml
Product group Antibodies
References
GeneTex
SUMO1 antibody [4D12]GTX00701
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Primate, Rat
TargetSUMO1
  • SizePrice
ICC/IF analysis of various samples using GTX00702 HB EGF antibody [4G10]. Vero-H : Vero cells overexpressing human HB-EGF protein Vero-mH : Vero cells overexpressing mouse HB-EGF protein Fixation : 4% PFA
Product group Antibodies
References
GeneTex
HB EGF antibody [4G10] - Overexpression ValidatedGTX00702
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking
ReactivityHuman
TargetHBEGF
  • SizePrice
ICC/IF analysis of HeLa cells using GTX00703 VRK1 antibody [5D1]. Upper panel : Interphase cells Middle and lower panel : Metaphase cells Dilution : 1:100 Fixation : Methanol
Product group Antibodies
GeneTex
VRK1 antibody [5D1] - KO/KD, Orthogonal ValidatedGTX00703
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetVRK1
  • SizePrice
ICC/IF analysis of GM0637 cells irradiated with UV at 20 J/m2 using GTX00704 XPA antibody [5F12]. Dilution : 1:100
Product group Antibodies
References
GeneTex
XPA antibody [5F12] - Orthogonal ValidatedGTX00704
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, Neutralisation/Blocking
ReactivityHuman
TargetXPA
  • SizePrice
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