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Phosphotyrosine Detection: Lysates of pervanadate-treated A431 cells were probed with: lane 1: mab 2A5 (1 g/ml), lane 2: mab 2C8 (1 g/ml), lane 3: mab 3B12 (1 g/ml), lane 4: mab 9H8 (1 g/ml), lane 5: mab 16F4 (1 g/ml), lane 6: mab 9F1 (1 g/ml).
Product group Antibodies
OriGene
Phosphotyrosine 7x sampler kit (incl. pos. control) Mouse Polyclonal AntibodyAM00129PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse, Rat
  • SizePrice
Transactivation of PYK2 Whole cell lysates of serum starved HepG2 tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab PYK2-14F6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1:Co; lane 2: serum; lane 3: EGF; lane 4: H2O2;lane 5: Anisomycin; lane 6: Sorbit; lane 7: Arsen; lane 8:Ceramide.
Product group Antibodies
OriGene
PYK2 (PTK2B) pTyr402 Mouse Monoclonal Antibody [Clone ID: 14F6]AM00130PU-N
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPTK2B
  • SizePrice
Product group Antibodies
OriGene
RAF1 pSer621 Mouse Monoclonal Antibody [Clone ID: 6B4]AM00131PU-N
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetRAF1
  • SizePrice
Immunoblot Analysis A431 cell lysate probed with mab C-Raf (PBB-1) at 0.1 ug/ml.
Product group Antibodies
OriGene
RAF1 Mouse Monoclonal Antibody [Clone ID: PBB-1]AM00132PU-N
ApplicationsWestern Blot
ReactivityHuman
TargetRAF1
  • SizePrice
Detection of endogenous SAPK2alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK2a-13D5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA-MB231; lane 7: T47D
Product group Antibodies
OriGene
p38 (MAPK14) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 13D5]AM00136PU-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetMAPK14
  • SizePrice
Detection of endogenous SAPK2alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK2a-20B11 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA-MB231; lane 7: T47D
Product group Antibodies
OriGene
p38 (MAPK14) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 20B11]AM00137PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetMAPK14
  • SizePrice
Detection of endogenous SAPK2 delta Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK2d-5H7 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: MDA-MB 231; lane 2: MDA-MB 468; lane 3: MCF-7; lane 4: T47D; lane 5: SW480; lane 6: SW620
Product group Antibodies
OriGene
SAPK4 (MAPK13) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5H7]AM00138PU-N
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetMAPK13
  • SizePrice
Detection of Endogenous Scramblase 1. Whole cell lysates of serum starved tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab Scram1-1E9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK-293 Lane 4: SY5Y Lane 5: Jurkat Lane 6: U937 Lane 7: K562 Lane 8: THP
Product group Antibodies
OriGene
Scramblase 1 (PLSCR1) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1E9]AM00139PU-N
ApplicationsWestern Blot
ReactivityHuman
TargetPLSCR1
  • SizePrice
Detection of endogenous shc: Whole cell lysates of serum starved tumor cells (ca. 20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab shc-11F6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A; lane 9: 3T3
Product group Antibodies
OriGene
SHC (SHC1) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11F6]AM00140PU-N
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetSHC1
  • SizePrice
Phosphospecificity Whole cell extracts of control (co), EGF-stimulated (EGF) or pervanadate-treated (VH) A549 tumor cells were applied to SDS-PAGE (ca. 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab shc-1E3 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
SHC (SHC1) pTyr239/240 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1E3]AM00141PU-N
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse
TargetSHC1
  • SizePrice
Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab shc-15E11 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
SHC (SHC1) pTyr317 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 15E11]AM00142PU-N
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse
TargetSHC1
  • SizePrice
Detection of endogenous p66shc: Whole cell lysates of serum starved tumor cells (ca. 20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab shc/p66-24E4 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT 93 Lane 8: Neuro 2A Lane 9: 3T3
Product group Antibodies
OriGene
SHC (SHC1) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 24E4]AM00143PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse, Rat
TargetSHC1
  • SizePrice
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