More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/SHC (SHC1) pTyr317 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 15E11]
Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab shc-15E11 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab shc-15E11 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab shc-15E11 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).

SHC (SHC1) pTyr317 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 15E11]

AM00142PU-N
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSHC1
100 ug
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    SHC (SHC1) pTyr317 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 15E11]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    15E11
  • Gene ID6464
  • Target name
    SHC1
  • Target description
    SHC adaptor protein 1
  • Target synonyms
    SHC, SHCA, SHC-transforming protein 1, SH2 domain protein C1, SHC (Src homology 2 domain containing) transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP29353
  • Protein Name
    SHC-transforming protein 1
  • Scientific Description
    SHC (SHC1) pTyr317 (incl. pos. control) mouse monoclonal antibody, clone 15E11, Purified
  • Storage Instruction
    -20°C,-80°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of HepG2 cells using Shc Polyclonal Antibody
Product group Antibodies
Cusabio
SHC1 AntibodyCSB-PA004082
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSHC1
  • SizePrice
Product group Antibodies
GeneTex
SHC1 antibody [N3C2], InternalGTX113267
ApplicationsWestern Blot
TargetSHC1
  • SizePrice
Product group Antibodies
MedChem Express
SHC Antibody (YA2394, Rabbit)HY-P82649
TargetSHC1
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-Shc1 Antibody, Affinity PurifiedA302-019A
ApplicationsImmunoPrecipitation
TargetSHC1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-SHC1 Antibody144-07725
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetSHC1
  • SizePrice
Figure 1. Western blot analysis of SHC using anti-SHC antibody (PB9391). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysate, Lane 2: human Hela whole cell lysate, Lane 3: human HepG2 whole cell lysate, Lane 4: human Jurkat whole cell lysate, Lane 5: human K562 whole cell lysate, Lane 6: human THP-1 whole cell lysate, Lane 7: rat C6 whole cell lysate, Lane 8: mouse thymus tissue lysate, Lane 9: mouse RAW246.7 whole cell lysate, Lane 10: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHC antigen affinity purified polyclonal antibody (Catalog # PB9391) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for SHC at approximately 46, 52, 66KD. The expected band size for SHC are at 46, 52, 66KD.
Product group Antibodies
Boster Bio
Anti-SHC/SHC1 Antibody Picoband(r)PB9391-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
TargetSHC1
  • SizePrice
Anti-SHC Antibody
Product group Antibodies
Antibodies.com
Anti-SHC AntibodyA16912
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • SizePrice
Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.
Product group Antibodies
Atlas Antibodies
Anti-SHC1 AntibodyHPA001844
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetSHC1
  • SizePrice
Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Rabbit Anti phospho-SHC(Ser36) Polyclonal Antibody, Unconjugated (bs-3410R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
References
Bioss
phospho-SHC1 (Ser36) Polyclonal AntibodyBS-3410R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetSHC1
  • SizePrice
Product group Antibodies
Biomatik
SHC-Transforming Protein 1 (SHC1) Polyclonal AntibodyCAU22754
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetSHC1
  • SizePrice