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ProductsBack/SHC (SHC1) pTyr317 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 15E11]
Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab shc-15E11 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab shc-15E11 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab shc-15E11 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).

SHC (SHC1) pTyr317 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 15E11]

AM00142PU-N
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetSHC1
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SHC (SHC1) pTyr317 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 15E11]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    15E11
  • Gene ID6464
  • Target name
    SHC1
  • Target description
    SHC adaptor protein 1
  • Target synonyms
    SH2 domain protein C1; SHC; SHC (Src homology 2 domain containing) transforming protein 1; SHCA; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP29353
  • Protein Name
    SHC-transforming protein 1
  • Scientific Description
    SHC (SHC1) pTyr317 (incl. pos. control) mouse monoclonal antibody, clone 15E11, Purified
  • Storage Instruction
    -20°C,-80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SHC using anti-SHC antibody (PB9391). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysate, Lane 2: human Hela whole cell lysate, Lane 3: human HepG2 whole cell lysate, Lane 4: human Jurkat whole cell lysate, Lane 5: human K562 whole cell lysate, Lane 6: human THP-1 whole cell lysate, Lane 7: rat C6 whole cell lysate, Lane 8: mouse thymus tissue lysate, Lane 9: mouse RAW246.7 whole cell lysate, Lane 10: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHC antigen affinity purified polyclonal antibody (Catalog # PB9391) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for SHC at approximately 46, 52, 66KD. The expected band size for SHC are at 46, 52, 66KD.
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