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Immunoblot Analysis: HeLa cells were cultured under serum-free conditions for 24h and subsequently stimulated with 10 ng/ml EGF. Cells were lysed with RIPA buffer and shc immunoprecipitated with polyclonal anti-shc (Transduction Labs). Immunoprecipitates were separated by SDS-PAGE. Immunoblots were developed using mab shc/p66-6E10 at 1 ug/ml.
Product group Antibodies
OriGene
SHC (SHC1) pSer36 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6E10]AM00144PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse
TargetSHC1
  • SizePrice
Product group Antibodies
OriGene
SRC pTyr416 Mouse Monoclonal Antibody [Clone ID: 9A6]AM00145BT-N
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse
TargetSRC
  • SizePrice
Phosphospecificity: Whole cell extracts of control (co) and pervanadate stimulated HEK-293 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with mab src 9A6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: co; Lane 2: pervanadate
Product group Antibodies
OriGene
SRC pTyr416 Mouse Monoclonal Antibody [Clone ID: 9A6]AM00145PU-N
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse
TargetSRC
  • SizePrice
Detection of endogenous Src: Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with AM00146PU-N Src antibody Clone 11F1 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D
Product group Antibodies
OriGene
SRC (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11F1]AM00146PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetSRC
  • SizePrice
Phosphospecificity Whole cell extracts of control (1), EGF stimulated (2) or pervanadate treated (3) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab STAT1-12C5 (0.5
Product group Antibodies
OriGene
STAT1 pSer727 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12C5]AM00147PU-N
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetSTAT1
  • SizePrice
Product group Antibodies
OriGene
STAT3 pTyr705 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9E12]AM00148BT-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse
TargetSTAT3
  • SizePrice
Product group Antibodies
OriGene
STAT3 pTyr705 Mouse Monoclonal Antibody [Clone ID: 9E12]AM00148FC-N
ApplicationsImmunoFluorescence
ReactivityCanine, Human, Mouse
TargetSTAT3
  • SizePrice
Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) HEPG2 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab STAT3-9E12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
STAT3 pTyr705 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9E12]AM00148PU-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse
TargetSTAT3
  • SizePrice
Phosphospecificity Whole cell extracts of control (1), EGF stimulated (2) or pervanadate treated (3) A 431 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab STAT3-23G5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 0 sec).
Product group Antibodies
OriGene
STAT3 pSer727 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 23G5]AM00149PU-N
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetSTAT3
  • SizePrice
Activation of STAT5 A/B in MCF-7 cells Whole cell lysates of untreated (lane 1) and pervanadate-treated (lane 2) MCF-7 cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab STAT5-5G4 at 0.5 ug/ml for 1h at 15-22°C and developed by ECL (exposure time: 5 sec).
Product group Antibodies
OriGene
STAT5A pTyr695/699 Mouse Monoclonal Antibody [Clone ID: 5G4]AM00150PU-N
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSTAT5A
  • SizePrice
Phosphospecificity Whole cell lysates of untreated (Lane 1) and IL4-treated (Lane 2) HepG2 cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with STAT6 antibody clone 16E12 at 1ug/ml for 1h at 15-22°C and developed by ECL (exposure time: 3min).
Product group Antibodies
OriGene
STAT6 pTyr641 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 16E12]AM00151PU-N
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSTAT6
  • SizePrice
Detection of Endogenous STAT6: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblots was probed with mab STAT6-8C12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431. Lane 2: SW480 Lane 3: SW620 Lane 4: HT29. Lane 5: MCF7. Lane 6: MDA-MB231 Lane 7: T47D
Product group Antibodies
OriGene
STAT6 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 8C12]AM00152PU-N
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human
TargetSTAT6
  • SizePrice
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