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ProductsBack/SRC (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11F1]
Detection of endogenous Src: Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with AM00146PU-N Src antibody Clone 11F1 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D
Detection of endogenous Src: Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with AM00146PU-N Src antibody Clone 11F1 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D
Detection of endogenous Src: Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with AM00146PU-N Src antibody Clone 11F1 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D

SRC (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11F1]

AM00146PU-N
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSRC
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SRC (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11F1]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    11F1
  • Gene ID6714
  • Target name
    SRC
  • Target description
    SRC proto-oncogene, non-receptor tyrosine kinase
  • Target synonyms
    ASV, SRC1, THC6, c-SRC, p60-Src, proto-oncogene tyrosine-protein kinase Src, proto-oncogene c-Src, protooncogene SRC, Rous sarcoma, tyrosine kinase pp60c-src, tyrosine-protein kinase SRC-1, v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP12931
  • Protein Name
    Proto-oncogene tyrosine-protein kinase Src
  • Scientific Description
    SRC (N-term) (incl. pos. control) mouse monoclonal antibody, clone 11F1, Purified
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Src using anti-Src antibody (PB9059). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Src antigen affinity purified polyclonal antibody (Catalog # PB9059) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Src at approximately 60 kDa. The expected band size for Src is at 60 kDa.
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