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Western blot analysis of MBP using anti-MBP antibody (PA1050). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Mouse Brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBP antigen affinity purified polyclonal antibody (Catalog # PA1050) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
References
Boster Bio
Anti-Myelin Basic Protein/MBP Antibody Picoband(r)PA1050
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHamster, Mouse, Rat
TargetMBP
IHC analysis of MIF using anti-MIF antibody (PA1052). MIF was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MIF Antibody (PA1052) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-MIF Antibody Picoband(r)PA1052
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityEquine, Human, Mouse, Rat
TargetMIF
Anti-Morg1 antibody, PA1053, Western blotting WB: Rat Brain Tissue Lysate
Product group Antibodies
Boster Bio
Anti-Morg1/WDR83 Antibody Picoband(r)PA1053
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityChicken, Human, Mouse, Rat
TargetWDR83
Western blot analysis of MPO using anti-MPO antibody (PA1054).
Product group Antibodies
References
Boster Bio
Anti-Myeloperoxidase/MPO Antibody Picoband(r)PA1054
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMPO
Anti-NGF antibody, PA1056, Western blotting WB: Rat Brain Tissue Lysate
Product group Antibodies
References
Boster Bio
Anti-Beta-nerve growth factor NGF Antibody Picoband(r)PA1056
ApplicationsWestern Blot
ReactivityBovine, Human, Mouse, Rat
TargetNGF
Product group Antibodies
Boster Bio
Anti-NMDAR2A/GRIN2A Antibody Picoband(r)PA1058-1
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGRIN2A
Anti-NMDAR2B antibody, PA1059-1, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: Mouse Brain Tissue Lysate Lane 3: U87 Cell Lysate
Product group Antibodies
Boster Bio
Anti-NMDAR2B/GRIN2B Antibody Picoband(r)PA1059-1
ApplicationsWestern Blot
ReactivityCanine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetGRIN2B
Anti-NMDAR2B antibody, PA1059, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Brain Tissue Lysate Lane 3: Mouse Brain Tissue Lysate Lane 4: Mouse Brain Tissue Lysate
Product group Antibodies
Boster Bio
Anti-NMDAR2B/GRIN2B Antibody Picoband(r)PA1059
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetGRIN2B
Western blot analysis of TYMS using anti-TYMS antibody in HeLa cell lysate. (M04320-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYMS antigen affinity purified polyclonal antibody (Catalog # M04320-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Thymidylate Synthase TYMS Monoclonal AntibodyM04320-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of Urokinase expression in LnCaP cell lysate (M04352). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAU monoclonal antibody (Catalog # M04352) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAU
Product group Antibodies
Boster Bio
Anti-Urokinase Rabbit Monoclonal AntibodyM04352
ApplicationsWestern Blot, ImmunoHistoChemistry
Western blot analysis of Thymidine Kinase 1 expression in Molt-4 cell lysate (M04850). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TK1 monoclonal antibody (Catalog # M04850) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TK1
Product group Antibodies
Boster Bio
Anti-Thymidine Kinase 1 Rabbit Monoclonal AntibodyM04850
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of Aldolase using anti-Aldolase antibody in A549 cell lysate (M05022). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aldolase antigen affinity purified polyclonal antibody (Catalog # M05022) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Aldolase ALDOA Monoclonal AntibodyM05022
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
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