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Western blot analysis of CS using anti-CS antibody (M05128) in (1) HeLa cell lysate; (1) Mouse spleen lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CS antigen affinity purified polyclonal antibody (Catalog # M05128) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Citrate synthetase CS Monoclonal AntibodyM05128
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of Caspase-14 expression in Human skin lysate (M05143). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP14 monoclonal antibody (Catalog # M05143) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP14
Product group Antibodies
Boster Bio
Anti-Caspase-14 Rabbit Monoclonal AntibodyM05143
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of Caspase 5 expression in HeLa cell lysate (M05259). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP5 monoclonal antibody (Catalog # M05259) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Caspase 5 CASP5 Rabbit Monoclonal AntibodyM05259
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of intestinal alkaline phosphatase expression in HeLa cell lysate.
Product group Antibodies
Boster Bio
Anti-Alkaline phosphatase ALPI Rabbit Monoclonal AntibodyM06115
ApplicationsWestern Blot, ImmunoHistoChemistry
Western blot analysis of HDAC11 expression in (1) Jurkat cell lysate;(2) MCF7 cell lysate (M06209). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC11 monoclonal antibody (Catalog # M06209) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC11
Product group Antibodies
Boster Bio
Anti-HDAC11/Histone Deacetylase 11 Rabbit Monoclonal AntibodyM06209
ApplicationsImmunoPrecipitation, Western Blot
Western blot analysis of INPP4B expression in MCF-7 cell lysate.
Product group Antibodies
References
Boster Bio
Anti-INPP4B/Type Ii 4 Phosphatase Rabbit Monoclonal AntibodyM06336
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Immunohistochemical analysis of paraffin-embedded human astrocytoma, using PI 3 Kinase p55 gamma Antibody(M06707) PIK3R3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PIK3R3 Antibody (M06707)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-PI 3 Kinase p55 gamma PIK3R3 Rabbit Monoclonal AntibodyM06707
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Western blot analysis of Mast Cell Tryptase expression in Human tonsil lysate.
Product group Antibodies
Boster Bio
Anti-Mast Cell Tryptase Rabbit Monoclonal AntibodyM08039
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Western blot analysis of MAP kinase,activated using anti-MAP kinase,activated antibody (MA1055).
Product group Antibodies
References
Boster Bio
Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT)MA1055
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1)MA1072
ApplicationsWestern Blot
IHC analysis of Tyrosine Hydroxylase using anti-Tyrosine Hydroxylase antibody (MA1100). Tyrosine Hydroxylase was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Tyrosine Hydroxylase Antibody (MA1100) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Product group Antibodies
References
Boster Bio
Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16)MA1100
ApplicationsWestern Blot, ImmunoHistoChemistry
Western blot analysis of Firefly Luciferase Antibody - N-terminal expression in (1) Firefly Luciferase transfected 293T cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate.
Product group Antibodies
Boster Bio
Anti-Firefly Luciferase Rabbit Monoclonal AntibodyMT0021-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
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