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Western blot analysis of Firefly Luciferase Antibody expression in (1) Firefly Luciferase transfected 293T cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Western blot analysis of Renilla Luciferase expression in (1) Renilla Luciferase transfected 293 cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate.
Product group Antibodies
References
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Western blot analysis of Phospho-Glycogen synthase 1 (S641) expression in HeLa cell lysate (P03512-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GYS1 monoclonal antibody (Catalog # P03512-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
All lanes: Anti ALPL (PA1004-1) at 0.5ug/ml Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug Lane 2: Rat Brain Tissue Lysate at 50ug Predicted bind size: 57KD Observed bind size: 57KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
All lanes: Anti ALPL (PA1004-2) at 0.5ug/ml Lane 1: Human Placenta Tissue Lysate at 50ug Lane 2: HT1080 Whole Cell Lysate at 40ug Lane 3: JURKAT Whole Cell Lysate at 40ug Predicted bind size: 57KD Observed bind size: 57KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
Anti-Alkaline Phosphatase antibody, PA1004, Western blotting All lanes: Anti ALPL (PA1004) at 0.5ug/ml WB: HT1080 Whole Cell Lysate at 40ug Predicted bind size: 57KD Observed bind size: 57KD
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
Anti-Caspase-2 antibody, PA1113, Western blotting Lane 1: CEM Cell Lysate Lane 2: SMMC Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
Anti-Topoisomerase II alpha antibody, PA1127, Western blotting All lanes: Anti Topoisomerase II alpha (PA1127) at 0.5ug/ml Lane 1: HELA Whole Cell Lysate at 40ug Lane 2: JURKAT Whole Cell Lysate at 40ug Predicted bind size: 174KD Observed bind size: 174KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
Anti-Caspase-3(P10), PA1302-1, IHC(P) IHC(P): Human Intestinal Cancer Tissue
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Anti-Caspase-8(P18) antibody, PA1308, Western blotting All lanes: Anti Caspase-8(P18) (PA1308) at 0.5ug/ml Lane 1: Rat Thymus Tissue Lysate at 50ug Lane 2: Rat Liver Tissue Lysate at 50ug Lane 3: MCF-7 Whole Cell Lysate at 40ug Lane 4: HELA Whole Cell Lysate at 40ug Lane 5: SMMC Whole Cell Lysate at 40ug Predicted bind size: 55KD Observed bind size: 55KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
Anti-PI 3 Kinase p85 alpha antibody, PA1410, Western blotting Lane 1: MCF-7 Cell Lysate Lane 2: HELA Cell Lysate Lane 3: COLO-320 whole cell lysate Lane 4: SW620 Cell Lysate
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot
Western blot analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (PA1425).
Product group Antibodies
Boster Bio
ApplicationsWestern Blot