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ProductsBack/MAT1A + MAT2A antibody [HL1679]
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MAT1A + MAT2A antibody [HL1679] (GTX637278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MAT1A + MAT2A antibody [HL1679] (GTX637278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MAT1A + MAT2A antibody [HL1679] (GTX637278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MAT1A + MAT2A antibody [HL1679]

GTX637278
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityDrosophila, Human, Mouse
TargetMAT1A
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Overview

  • Supplier
    GeneTex
  • Product Name
    MAT1A + MAT2A antibody [HL1679]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL1679
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4143
  • Target name
    MAT1A
  • Target description
    methionine adenosyltransferase 1A
  • Target synonyms
    MAT, MATA1, SAMS, SAMS1, S-adenosylmethionine synthase isoform type-1, MAT 1, MAT-I/III, adoMet synthase 1, adoMet synthetase 1, methionine adenosyltransferase 1, methionine adenosyltransferase I, alpha, methionine adenosyltransferase I/III
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31153
  • Protein Name
    S-adenosylmethionine synthase isoform type-2
  • Reactivity
    Drosophila, Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAT1A using anti-MAT1A antibody (A04203-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAT1A antigen affinity purified polyclonal antibody (Catalog # A04203-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAT1A at approximately 55 kDa. The expected band size for MAT1A is at 44 kDa.
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