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WB analysis of various sample lysates using GTX64527 MAT2A antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64527 MAT2A antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64527 MAT2A antibody. Dilution : 1:1000 Loading : 25microg per lane

MAT2A antibody

GTX64527
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetMAT2A
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Overview

  • Supplier
    GeneTex
  • Product Name
    MAT2A antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4144
  • Target name
    MAT2A
  • Target description
    methionine adenosyltransferase 2A
  • Target synonyms
    MATA2, MATII, SAMS2, S-adenosylmethionine synthase isoform type-2, MAT 2, MAT-II, adoMet synthase 2, adoMet synthetase 2, methionine adenosyltransferase 2, methionine adenosyltransferase II, alpha, testicular tissue protein Li 121
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31153
  • Protein Name
    S-adenosylmethionine synthase isoform type-2
  • Scientific Description
    The protein encoded by this gene catalyzes the production of S-adenosylmethionine (AdoMet) from methionine and ATP. AdoMet is the key methyl donor in cellular processes. [provided by RefSeq, Jun 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAT2A using anti-MAT2A antibody (A04557-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAT2A antigen affinity purified polyclonal antibody (Catalog # A04557-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAT2A at approximately 50 kDa. The expected band size for MAT2A is at 44 kDa.
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