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IP analysis of HeLa cell lysate using GTX33310 MCM5 antibody. Antibody amount : 3microg / 300microg lysate Dilution : 1:1000
IP analysis of HeLa cell lysate using GTX33310 MCM5 antibody. Antibody amount : 3microg / 300microg lysate Dilution : 1:1000
IP analysis of HeLa cell lysate using GTX33310 MCM5 antibody. Antibody amount : 3microg / 300microg lysate Dilution : 1:1000

MCM5 antibody

GTX33310
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetMCM5
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Overview

  • Supplier
    GeneTex
  • Product Name
    MCM5 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000 - 1:3000. ICC/IF: 1:50 - 1:200. IP: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4174
  • Target name
    MCM5
  • Target description
    minichromosome maintenance complex component 5
  • Target synonyms
    CDC46; CDC46 homolog; DNA replication licensing factor MCM5; MCM5 minichromosome maintenance deficient 5, cell division cycle 46; MGORS8; minichromosome maintenance deficient 5 (cell division cycle 46); P1-CDC46
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP33992
  • Protein Name
    DNA replication licensing factor MCM5
  • Scientific Description
    The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Metabolic clogging of mannose triggers dNTP loss and genomic instability in human cancer cells.
    Read more

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Figure 1. Western blot analysis of MCM5 using anti-MCM5 antibody (A03642). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM5 antigen affinity purified polyclonal antibody (Catalog # A03642) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM5 at approximately 100KD. The expected band size for MCM5 is at 82KD.
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