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ProductsBack/MCM7 antibody [AT118H7]
WB analysis of various samples using GTX57715 MCM7 antibody. Lane 1 : HeLa whole cell lysate Lane 2 : Jurkat whole cell lysate Lane 3 : K562 whole cell lysate Loading : 40 μg Dilution : 1:1000
WB analysis of various samples using GTX57715 MCM7 antibody. Lane 1 : HeLa whole cell lysate Lane 2 : Jurkat whole cell lysate Lane 3 : K562 whole cell lysate Loading : 40 μg Dilution : 1:1000
WB analysis of various samples using GTX57715 MCM7 antibody. Lane 1 : HeLa whole cell lysate Lane 2 : Jurkat whole cell lysate Lane 3 : K562 whole cell lysate Loading : 40 μg Dilution : 1:1000

MCM7 antibody [AT118H7]

GTX57715
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetMCM7
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Overview

  • Supplier
    GeneTex
  • Product Name
    MCM7 antibody [AT118H7]
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT118H7
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4176
  • Target name
    MCM7
  • Target description
    minichromosome maintenance complex component 7
  • Target synonyms
    CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, PPP1R104, DNA replication licensing factor MCM7, CDC47 homolog, homolog of S. cerevisiae Cdc47, minichromosome maintenance deficient 7, protein phosphatase 1, regulatory subunit 104
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP33993
  • Protein Name
    DNA replication licensing factor MCM7
  • Scientific Description
    The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MCM7 using anti-MCM7 antibody (PB9261). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM7 antigen affinity purified polyclonal antibody (Catalog # PB9261) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM7 at approximately 81 kDa. The expected band size for MCM7 is at 81 kDa.
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