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Cross-reactivity assessment of MCM7 Mouse Recombinant Antibody AE00255 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
Cross-reactivity assessment of MCM7 Mouse Recombinant Antibody AE00255 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
Cross-reactivity assessment of MCM7 Mouse Recombinant Antibody AE00255 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.

MCM7 Recombinant Antibody AE00255

AE00255
Aeonian Biotech
TargetMCM7
Product group Antibodies
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Overview

  • Supplier
    Aeonian Biotech
  • Product Name
    MCM7 Recombinant Antibody AE00255
  • Delivery Days Customer
    9
  • Applications Supplier
    IHC, PA, WB
  • Certification
    Research Use Only
  • Clonality
    Recombinant
  • Clone ID
    rMCM7/1468
  • Conjugate
    Unconjugated
  • Gene ID4176
  • Target name
    MCM7
  • Target description
    minichromosome maintenance complex component 7
  • Target synonyms
    CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, PPP1R104, DNA replication licensing factor MCM7, CDC47 homolog, homolog of S. cerevisiae Cdc47, minichromosome maintenance deficient 7, protein phosphatase 1, regulatory subunit 104
  • Host
    Mouse
  • Isotype
    IgG1 kappa
  • Protein IDP33993
  • Protein Name
    DNA replication licensing factor MCM7
  • Scientific Description
    MCM7 Recombinant Antibody AE00255
  • Shelf life instruction
    Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
  • Reactivity Supplier
    Human
  • Storage Instruction
    2-8°C
  • UNSPSC
    12352203

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of MCM7 using anti-MCM7 antibody (PB9261). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM7 antigen affinity purified polyclonal antibody (Catalog # PB9261) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM7 at approximately 81 kDa. The expected band size for MCM7 is at 81 kDa.
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