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MDH1 Rabbit mAb

A9673
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMDH1
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Overview

  • Supplier
    ABclonal
  • Product Name
    MDH1 Rabbit mAb
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID4190
  • Target name
    MDH1
  • Target description
    malate dehydrogenase 1
  • Target synonyms
    DEE88, EIEE88, HEL-S-32, KAR, MDH-s, MDHA, MGC:1375, MOR2, malate dehydrogenase, cytoplasmic, malate dehydrogenase, peroxisomal, aromatic alpha-keto acid reductase, cytosolic malate dehydrogenase, diiodophenylpyruvate reductase, epididymis secretory protein Li 32, malate dehydrogenase 1, NAD (soluble), soluble malate dehydrogenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40925
  • Protein Name
    Malate dehydrogenase, cytoplasmic
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MDH1 using anti-MDH1 antibody (A04262-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDH1 antigen affinity purified polyclonal antibody (Catalog # A04262-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDH1 at approximately 36 kDa. The expected band size for MDH1 is at 59 kDa.
Product group Antibodies
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ReactivityHuman, Mouse, Rat
TargetMDH1
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MDH1 Polyclonal AntibodyCAC15653
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Lane 1: MOLT-4 cell lysates; Lane 2: Jurkat cell lysates probed with MDH1 Polyclonal Antibody, Unconjugated (bs-3996R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
References
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ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetMDH1
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Western Blot Positive WB detected in: HL60 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, LO2 whole cell lysate, NIH/3T3 whole cell lysate, RAW264.7 whole cell lysate, Mouse liver tissue All lanes: MDH1 antibody at 2.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37, 28, 39 kDa Observed band size: 37 kDa
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Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
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