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Western blot analysis of extracts from 293 and Hela cells using MDM2 Antibody.
Western blot analysis of extracts from 293 and Hela cells using MDM2 Antibody.
Western blot analysis of extracts from 293 and Hela cells using MDM2 Antibody.

MDM2 Antibody

CSB-PA729984
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMDM2
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Overview

  • Supplier
    Cusabio
  • Product Name
    MDM2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4193
  • Target name
    MDM2
  • Target description
    MDM2 proto-oncogene
  • Target synonyms
    ACTFS, HDMX, LSKB, hdm2, E3 ubiquitin-protein ligase Mdm2, MDM2 oncogene, E3 ubiquitin protein ligase, MDM2 proto-oncogene, E3 ubiquitin protein ligase, Mdm2, p53 E3 ubiquitin protein ligase homolog, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein, double minute 2, human homolog of; p53-binding protein, oncoprotein Mdm2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00987
  • Protein Name
    E3 ubiquitin-protein ligase Mdm2
  • Scientific Description
    MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 . Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 . Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 . Haupt, Y. et al. (1997) Nature 387, 296-299. Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603. Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981. Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MDM2 using anti-MDM2 antibody (A00054-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: rat RH-35 whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates, Lane 5: mouse NTH/3T3 whole cell lysates, Lane 6: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDM2 antigen affinity purified polyclonal antibody (Catalog # A00054-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDM2 at approximately 90KD. The expected band size for MDM2 is at 90KD.
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