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ProductsBack/MDM2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1B4]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDM2 (RC219518, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MDM2. Positive lysates LY400855 (100ug) and LC400855 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDM2 (RC219518, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MDM2. Positive lysates LY400855 (100ug) and LC400855 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDM2 (RC219518, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MDM2. Positive lysates LY400855 (100ug) and LC400855 (20ug) can be purchased separately from OriGene.

MDM2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1B4]

TA801787AM
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetMDM2
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    MDM2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1B4]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1B4
  • Gene ID4193
  • Target name
    MDM2
  • Target description
    MDM2 proto-oncogene
  • Target synonyms
    ACTFS, HDMX, LSKB, hdm2, E3 ubiquitin-protein ligase Mdm2, MDM2 oncogene, E3 ubiquitin protein ligase, MDM2 proto-oncogene, E3 ubiquitin protein ligase, Mdm2, p53 E3 ubiquitin protein ligase homolog, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein, double minute 2, human homolog of; p53-binding protein, oncoprotein Mdm2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ00987
  • Protein Name
    E3 ubiquitin-protein ligase Mdm2
  • Scientific Description
    MDM2 mouse monoclonal antibody,clone 1B4, Biotinylated
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MDM2 using anti-MDM2 antibody (A00054-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: rat RH-35 whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates, Lane 5: mouse NTH/3T3 whole cell lysates, Lane 6: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDM2 antigen affinity purified polyclonal antibody (Catalog # A00054-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDM2 at approximately 90KD. The expected band size for MDM2 is at 90KD.
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