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Western Blot analysis of 293T cells using MDMX Polyclonal Antibody
Western Blot analysis of 293T cells using MDMX Polyclonal Antibody
Western Blot analysis of 293T cells using MDMX Polyclonal Antibody

MDM4 Antibody

CSB-PA010135
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMDM4
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Overview

  • Supplier
    Cusabio
  • Product Name
    MDM4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4194
  • Target name
    MDM4
  • Target description
    MDM4 regulator of p53
  • Target synonyms
    BMFS6, HDMX, MDMX, MRP1, protein Mdm4, MDM4, p53 regulator, MDM4-related protein 1, Mdm4 p53 binding protein homolog, double minute 4, human homolog of; p53-binding protein, mdm2-like p53-binding protein, protein Mdmx
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15151
  • Protein Name
    Protein Mdm4
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Lane 1: Mouse Stomach lysates; Lane 2: Mouse Cerebrum lysates probed with MDM4 Polyclonal Antibody, Unconjugated (bs-7663R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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WB analysis of MCF cells with DNA damage using GTX00677 MDMX (phospho Ser367) antibody [#15]. MCF cells were preincubated with the proteasome inhibitor MG132 (20 microM) followed by exposed to DNA damaging agents adriamycin (3 microM) or etoposide (20 microM). The cell lysates were used for immunoprecipitation with anti-MdmX antibody and then analyzed by Western blotting.Induction of S367 phosphorylation after DNA damage is associated with increased binding of 14-3-3 to MdmX and accelerated MdmX degradation.
Product group Antibodies
References
GeneTex
MDMX (phospho Ser367) antibody [#15]GTX00677
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Rabbit anti-HdmX/MDM4 Antibody, Affinity PurifiedA300-287A
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Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
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Anti-MDM4 AntibodyHPA018919
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Figure 1. Western blot analysis of MDMX/MDM4 using anti-MDMX/MDM4 antibody (A01889-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDMX/MDM4 antigen affinity purified polyclonal antibody (Catalog # A01889-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDMX/MDM4 at approximately 55 kDa. The expected band size for MDMX/MDM4 is at 55 kDa.
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Anti-MDMX/MDM4 Antibody Picoband(r)A01889-2-CARRIER-FREE
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MDM4 / MDMX AntibodyLS-C408345
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TargetMDM4
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