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ProductsBack/MDM4 / MDMX Antibody

MDM4 / MDMX Antibody

LS-C408345
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetMDM4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MDM4 / MDMX Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 13kDa/14kDa/18kDa/49kDa/54kDa, while the observed MW by Western blot was 47kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) The predicted MW is 13kDa/14kDa/18kDa/49kDa/54kDa, while the observed MW by Western blot was 47kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4194
  • Target name
    MDM4
  • Target description
    MDM4 regulator of p53
  • Target synonyms
    BMFS6; double minute 4, human homolog of; p53-binding protein; HDMX; mdm2-like p53-binding protein; Mdm4 p53 binding protein homolog; MDM4 protein variant G; MDM4 protein variant Y; MDM4, p53 regulator; MDM4-related protein 1; MDMX; MRP1; protein Mdm4; protein Mdmx
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MDMX/MDM4 using anti-MDMX/MDM4 antibody (A01889-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDMX/MDM4 antigen affinity purified polyclonal antibody (Catalog # A01889-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDMX/MDM4 at approximately 55 kDa. The expected band size for MDMX/MDM4 is at 55 kDa.
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