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ME1 Antibody

CSB-PA010149
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetME1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ME1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4199
  • Target name
    ME1
  • Target description
    malic enzyme 1
  • Target synonyms
    HUMNDME; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)); malic enzyme 1, NADP(+)-dependent, cytosolic; malic enzyme 1, soluble; Malic enzyme, cytoplasmic; MES; NADP-dependent malic enzyme; NADP-ME; pyruvic-malic carboxylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48163
  • Protein Name
    NADP-dependent malic enzyme
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ME1 using anti-ME1 antibody (A03449-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ME1 antigen affinity purified polyclonal antibody (Catalog # A03449-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ME1 at approximately 64 kDa. The expected band size for ME1 is at 64 kDa.
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Immunohistochemistry analysis in human testis and kidney tissues using HPA006493 antibody. Corresponding ME1 RNA-seq data are presented for the same tissues.
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