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ME1 / Malate Dehydrogenase Antibody

LS-C667898
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetME1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ME1 / Malate Dehydrogenase Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western blot analysis of ME1 expression in MCF7 (A), mouse heart (B), mouse brain (C) whole cell lysates.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Western blot analysis of ME1 expression in MCF7 (A), mouse heart (B), mouse brain (C) whole cell lysates.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4199
  • Target name
    ME1
  • Target description
    malic enzyme 1
  • Target synonyms
    HUMNDME, MES, NADP-dependent malic enzyme, Malic enzyme, cytoplasmic, NADP-ME, malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)), malic enzyme 1, NADP(+)-dependent, cytosolic, malic enzyme 1, soluble, pyruvic-malic carboxylase
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ME1 using anti-ME1 antibody (A03449-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ME1 antigen affinity purified polyclonal antibody (Catalog # A03449-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ME1 at approximately 64 kDa. The expected band size for ME1 is at 64 kDa.
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