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ME2 Antibody

CSB-PA010151
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetME2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ME2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4200
  • Target name
    ME2
  • Target description
    malic enzyme 2
  • Target synonyms
    malate dehydrogenase (oxaloacetate-decarboxylating); malic enzyme 2, NAD(+)-dependent, mitochondrial; NAD-dependent malic enzyme, mitochondrial; NAD-ME; ODS1; pyruvic-malic carboxylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23368
  • Protein Name
    NAD-dependent malic enzyme, mitochondrial
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ME2 using anti-ME2 antibody (A01380-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human placenta tissue lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ME2 antigen affinity purified polyclonal antibody (Catalog # A01380-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ME2 at approximately 65KD. The expected band size for ME2 is at 65KD.
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