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Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ME2 Monoclonal Antibody, Unconjugated (bsm-51670M) at 1:25 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ME2 Monoclonal Antibody, Unconjugated (bsm-51670M) at 1:25 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ME2 Monoclonal Antibody, Unconjugated (bsm-51670M) at 1:25 overnight at 4°C, DAB staining.

ME2 Monoclonal Antibody

BSM-51670M
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetME2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    ME2 Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-4000), IHC-P(IHC-P=1:25), IHC-F(IHC-F=1:25), IF(IF=1:25)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    J9E1
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID4200
  • Target name
    ME2
  • Target description
    malic enzyme 2
  • Target synonyms
    malate dehydrogenase (oxaloacetate-decarboxylating); malic enzyme 2, NAD(+)-dependent, mitochondrial; NAD-dependent malic enzyme, mitochondrial; NAD-ME; ODS1; pyruvic-malic carboxylase
  • Host
    Mouse
  • Protein IDP23368
  • Protein Name
    NAD-dependent malic enzyme, mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ME2 using anti-ME2 antibody (A01380-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human placenta tissue lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ME2 antigen affinity purified polyclonal antibody (Catalog # A01380-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ME2 at approximately 65KD. The expected band size for ME2 is at 65KD.
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