More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/ME3 Antibody (aa38-87)

ME3 Antibody (aa38-87)

LS-C81250
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetME3
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ME3 Antibody (aa38-87)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Applications
    Western Blot
  • Applications Supplier
    WB ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10873
  • Target name
    ME3
  • Target description
    malic enzyme 3
  • Target synonyms
    malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)); malic enzyme 3, NADP(+)-dependent, mitochondrial; malic enzyme, NADP+-dependent, mitochondrial; mitochondrial NADP(+)-dependent malic enzyme 3; NADP-dependent malic enzyme, mitochondrial; NADP-ME; pyruvic-malic carboxylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
Anti-ME3 Antibody144-61203
ApplicationsWestern Blot
TargetME3
  • SizePrice
Product group Antibodies
Biomatik
ME3 Polyclonal AntibodyCAC14561
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetME3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ME3 Antibody (C-Terminus)LS-C368905
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetME3
  • SizePrice
Western Blot analysis of RAW264.7 cells using ME3 Polyclonal Antibody
Product group Antibodies
Cusabio
ME3 AntibodyCSB-PA006739
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetME3
  • SizePrice
Immunohistochemistry analysis in human endometrium and tonsil tissues using HPA038473 antibody. Corresponding ME3 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-ME3 AntibodyHPA038473
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetME3
  • SizePrice
Figure 1. Western blot analysis of ME3 using anti-ME3 antibody (A05164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS7 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 8: mouse heart tissue lysates, Lane 9: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ME3 antigen affinity purified polyclonal antibody (Catalog # A05164-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ME3 at approximately 67 kDa. The expected band size for ME3 is at 67 kDa.
Product group Antibodies
Boster Bio
Anti-ME3 Antibody Picoband(r)A05164-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetME3
  • SizePrice
Product group Antibodies
References
GeneTex
ME3 antibodyGTX130689
ApplicationsWestern Blot
TargetME3
  • SizePrice
Anti-ME3 Antibody
Product group Antibodies
Antibodies.com
Anti-ME3 AntibodyA99712
ApplicationsWestern Blot, ELISA
  • SizePrice