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MECOM Rabbit pAb

A5674
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetMECOM
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Overview

  • Supplier
    ABclonal
  • Product Name
    MECOM Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2122
  • Target name
    MECOM
  • Target description
    MDS1 and EVI1 complex locus
  • Target synonyms
    AML1-EVI-1, EVI1, KMT8E, MDS1, MDS1-EVI1, PRDM3, RUSAT2, histone-lysine N-methyltransferase MECOM, AML1-EVI-1 fusion protein, MDS1 and EVI1 complex locus protein EVI1, MDS1 and EVI1 complex locus protein MDS1, PR domain 3, ecotropic virus integration site 1 protein homolog, myelodysplasia syndrome-associated protein 1, oncogene EVI1, zinc finger protein Evi1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03112
  • Protein Name
    Histone-lysine N-methyltransferase MECOM
  • Reactivity
    Human, Rat
  • Reactivity Supplier Note
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EVI1/MECOM using anti-EVI1/MECOM antibody (A04590-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Skov3 whole cell lysates, Lane 2: human Hel whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EVI1/MECOM antigen affinity purified polyclonal antibody (Catalog # A04590-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EVI1/MECOM at approximately 138 kDa. The expected band size for EVI1/MECOM is at 138 kDa.
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