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WB analysis with the antibody against the TY1-tag (lane 1) and with the antibody against MeCP2 (lane 2), diluted 1:2,000 in TBST containing 3% milk powder.Figure 2B: WB was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 ug) with the antibody against human MeCP2, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.
WB analysis with the antibody against the TY1-tag (lane 1) and with the antibody against MeCP2 (lane 2), diluted 1:2,000 in TBST containing 3% milk powder.Figure 2B: WB was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 ug) with the antibody against human MeCP2, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.
WB analysis with the antibody against the TY1-tag (lane 1) and with the antibody against MeCP2 (lane 2), diluted 1:2,000 in TBST containing 3% milk powder.Figure 2B: WB was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 ug) with the antibody against human MeCP2, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.

MECP2 Rabbit Polyclonal Antibody

TA347245
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMECP2
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    MECP2 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID4204
  • Target name
    MECP2
  • Target description
    methyl-CpG binding protein 2
  • Target synonyms
    AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT, methyl-CpG-binding protein 2, meCp-2 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51608
  • Protein Name
    Methyl-CpG-binding protein 2
  • Scientific Description
    Rabbit Polyclonal MeCP2 Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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ChIP analysis of sheared chromatin from 10? U2OS cells using GTX60358 MECP2 antibody - ChIP grade. IgG (1 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ZMYND10 gene (used as a positive control) and CDC6 gene (used as a negative control). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
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Figure 1. Western blot analysis of MECP2 using anti-MECP2 antibody (A00047-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MECP2 antigen affinity purified polyclonal antibody (Catalog # A00047-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MECP2 at approximately 80 kDa. The expected band size for MECP2 is at 52 kDa.
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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MeCP2 Polyclonal Antibody, Unconjugated (bs-22098R) at 1:200 overnight at 4°C, DAB staining.
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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA013638ESR2HU at dilution of 1:100
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