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MED28 Polyclonal Antibody

RD79527A
Reddot Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMED28
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    MED28 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID80306
  • Target name
    MED28
  • Target description
    mediator complex subunit 28
  • Target synonyms
    1500003D12Rik, EG1, magicin, mediator of RNA polymerase II transcription subunit 28, endothelial-derived gene 1, endothelial-derived protein 1, mediator of RNA polymerase II transcription, subunit 28 homolog, merlin and Grb2-interacting cytoskeletal protein, tumor angiogenesis marker EG-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Med28 (mediator complex subunit 28), also known as EG1 (endothelial-derived protein 1), magicin (merlin and Grb2-interacting cytoskeletal protein) or tumor angiogenesis marker EG-1, is a 178 amino acid subunit of the Mediator complex that localizes to both nucleus and cytoplasm.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MED28 using anti-MED28 antibody (A08611-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED28 antigen affinity purified polyclonal antibody (Catalog # A08611-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED28 at approximately 24 kDa. The expected band size for MED28 is at 20 kDa.
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Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MED28 Polyclonal Antibody, Unconjugated (bs-18767R) at 1:400 overnight at 4°C, DAB staining.
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