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ProductsBack/MED28 Rabbit Polyclonal Antibody
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA365097 (MED28 Antibody) at dilution 1/25 (Original magnification: x200)
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA365097 (MED28 Antibody) at dilution 1/25 (Original magnification: x200)
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA365097 (MED28 Antibody) at dilution 1/25 (Original magnification: x200)

MED28 Rabbit Polyclonal Antibody

TA365097
OriGene
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetMED28
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    MED28 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID80306
  • Target name
    MED28
  • Target description
    mediator complex subunit 28
  • Target synonyms
    1500003D12Rik; EG1; endothelial-derived gene 1; endothelial-derived protein 1; magicin; mediator of RNA polymerase II transcription subunit 28; mediator of RNA polymerase II transcription, subunit 28 homolog; merlin and Grb2-interacting cytoskeletal protein; tumor angiogenesis marker EG-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H204
  • Protein Name
    Mediator of RNA polymerase II transcription subunit 28
  • Scientific Description
    MED28 rabbit polyclonal antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MED28 using anti-MED28 antibody (A08611-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED28 antigen affinity purified polyclonal antibody (Catalog # A08611-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED28 at approximately 24 kDa. The expected band size for MED28 is at 20 kDa.
Product group Antibodies
Boster Bio
Anti-MED28 Antibody Picoband(r)A08611-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA
TargetMED28
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IHC-P analysis of human brain tissue using GTX85388 MED28 antibody. Working concentration : 2.5 microg/ml
Product group Antibodies
References
GeneTex
MED28 antibodyGTX85388
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetMED28
  • SizePrice
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA826911(MED28 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
Product group Antibodies
Cusabio
MED28 AntibodyCSB-PA826911
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMED28
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Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MED28 Polyclonal Antibody, Unconjugated (bs-18767R) at 1:400 overnight at 4°C, DAB staining.
Product group Antibodies
Bioss Antibodies
MED28 Polyclonal AntibodyBS-18767R
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetMED28
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MED28 / Magicin AntibodyLS-C768112
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetMED28
  • SizePrice
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Product group Antibodies
Atlas Antibodies
Anti-MED28 AntibodyHPA035901
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
ReactivityHuman
TargetMED28
  • SizePrice
Anti-MED28 Antibody
Product group Antibodies
Antibodies.com
Anti-MED28 AntibodyA28706
ApplicationsWestern Blot
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Product group Antibodies
RayBiotech
Anti-MED28 Antibody144-63791
ApplicationsWestern Blot
TargetMED28
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