More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/MEK1 (MAP2K1) Rabbit Polyclonal Antibody
Western blot of human T47D cells showing specific immunolabeling of the ~45k MEK 1 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda phosphatase: I>> Ptase) The blot is identical to the control except that it was incubated in I>> Ptase (1200 units for 30 min) before being exposed to the MEK 1 Thr386 antibody. The immunolabeling of MEK 1 is completely eliminated by I>> Ptase.
Western blot of human T47D cells showing specific immunolabeling of the ~45k MEK 1 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda phosphatase: I>> Ptase) The blot is identical to the control except that it was incubated in I>> Ptase (1200 units for 30 min) before being exposed to the MEK 1 Thr386 antibody. The immunolabeling of MEK 1 is completely eliminated by I>> Ptase.
Western blot of human T47D cells showing specific immunolabeling of the ~45k MEK 1 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda phosphatase: I>> Ptase) The blot is identical to the control except that it was incubated in I>> Ptase (1200 units for 30 min) before being exposed to the MEK 1 Thr386 antibody. The immunolabeling of MEK 1 is completely eliminated by I>> Ptase.

MEK1 (MAP2K1) Rabbit Polyclonal Antibody

TA309168
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetMAP2K1
Price on request
100 ul
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    MEK1 (MAP2K1) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5604
  • Target name
    MAP2K1
  • Target description
    mitogen-activated protein kinase kinase 1
  • Target synonyms
    CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1, dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02750
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 1
  • Scientific Description
    Rabbit Anti-MEK1 (Thr386) Antibody (Phospho-Specific)
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Figure 1. Western blot analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1/MAP2K1 antigen affinity purified polyclonal antibody (Catalog # A00292) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1/MAP2K1 at approximately 45 kDa. The expected band size for MEK1/MAP2K1 is at 45 kDa.
Product group Antibodies
Boster Bio
Anti-MEK1/MAP2K1 Antibody Picoband(r)A00292-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Product group Antibodies
Biosynth
MEK1 antibody20R-2121
  • SizePrice
Product group Antibodies
RayBiotech
Anti-MAP2K1 Antibody144-00252
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Product group Antibodies
Biomatik
MAP2K1 Polyclonal AntibodyCAC14738
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetMAP2K1
  • SizePrice
Product group Antibodies
Assay Genie
MEK1 AntibodyCAB0252
ReactivityHuman
TargetMAP2K1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MEK1/2 Polyclonal Antibody, Unconjugated (bs-1041R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Product group Antibodies
References
Bioss
MEK1 + MEK2 Polyclonal AntibodyBS-1041R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetMAP2K1
  • SizePrice
Western Blot analysis of HuvEc cells using MEK-1 Polyclonal Antibody
Product group Antibodies
Cusabio
MAP2K1 AntibodyCSB-PA003217
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Product group Antibodies
Atlas Antibodies
Anti-MAP2K1 AntibodyHPA026430
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetMAP2K1
  • SizePrice
Product group Antibodies
MedChem Express
MEK1 Antibody (YA300, Rabbit)HY-P80217
TargetMAP2K1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MAP2K1 / MKK1 / MEK1 AntibodyLS-C405977
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice