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ProductsBack/MEK1 (MAP2K1) Rabbit Polyclonal Antibody
Western blot analysis of MEK1/2 in human brain tissue lysate with MEK1/2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of MEK1/2 in human brain tissue lysate with MEK1/2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of MEK1/2 in human brain tissue lysate with MEK1/2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

MEK1 (MAP2K1) Rabbit Polyclonal Antibody

TA349182
OriGene
ApplicationsWestern Blot
Product group Antibodies
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    MEK1 (MAP2K1) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ0VD16
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 1
  • Scientific Description
    Rabbit Polyclonal Anti-MEK1/2 Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1/MAP2K1 antigen affinity purified polyclonal antibody (Catalog # A00292) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1/MAP2K1 at approximately 45 kDa. The expected band size for MEK1/MAP2K1 is at 45 kDa.
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