Western blot analysis of extracts of C6 cells, using Phospho-MEK1-pS221 antibody (TA378245) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Western blot analysis of extracts of C6 cells, using Phospho-MEK1-pS221 antibody (TA378245) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
MEK1 (MAP2K1) Rabbit Polyclonal Antibody
TA378245
Overview
- SupplierOriGene
- Product NameMEK1 (MAP2K1) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID5604
- Target nameMAP2K1
- Target descriptionmitogen-activated protein kinase kinase 1
- Target synonymsCFC3; dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEK1; MEL; MKK1; PRKMK1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
- HostRabbit
- IsotypeIgG
- Scientific DescriptionPhospho-MEK1-pS221 Rabbit polyclonal Antibody
- ReactivityHuman, Mouse, Rat
- UNSPSC12352203