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Western blot (WB) analysis of MEK1 (Phospho-S298) polyclonal antibody at 1:1000 dilution Lane1:PMVEC whole cell lysate(40ug) Lane2:BV2 whole cell lysate(40ug) Lane3:Hela treated with λ-phosphatase whole cell lysate(40ug) Lane4:HeLa whole cell lysate(40ug)
Western blot (WB) analysis of MEK1 (Phospho-S298) polyclonal antibody at 1:1000 dilution Lane1:PMVEC whole cell lysate(40ug) Lane2:BV2 whole cell lysate(40ug) Lane3:Hela treated with λ-phosphatase whole cell lysate(40ug) Lane4:HeLa whole cell lysate(40ug)
Western blot (WB) analysis of MEK1 (Phospho-S298) polyclonal antibody at 1:1000 dilution Lane1:PMVEC whole cell lysate(40ug) Lane2:BV2 whole cell lysate(40ug) Lane3:Hela treated with λ-phosphatase whole cell lysate(40ug) Lane4:HeLa whole cell lysate(40ug)

MEK1 (MAP2K1) Rabbit Polyclonal Antibody

TA392531
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAP2K1
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    MEK1 (MAP2K1) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5604
  • Target name
    MAP2K1
  • Target description
    mitogen-activated protein kinase kinase 1
  • Target synonyms
    CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1, dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02750
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 1
  • Scientific Description
    MEK1 (Phospho-S298) polyclonal antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1/MAP2K1 antigen affinity purified polyclonal antibody (Catalog # A00292) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1/MAP2K1 at approximately 45 kDa. The expected band size for MEK1/MAP2K1 is at 45 kDa.
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