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MEK1 (phospho Thr386) Antibody

ORB1273520
Biorbyt
ApplicationsWestern Blot
Product group Antibodies
ReactivityRat
TargetMAP2K1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    MEK1 (phospho Thr386) Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
  • Applications
    Western Blot
  • Applications Supplier
    Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB. WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5604
  • Target name
    MAP2K1
  • Target description
    mitogen-activated protein kinase kinase 1
  • Target synonyms
    CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1, dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
  • Host
    Rabbit
  • Protein IDQ02750
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 1
  • Scientific Description
    MEK1 (phospho Thr386) Antibody
  • Reactivity
    Rat
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1/MAP2K1 antigen affinity purified polyclonal antibody (Catalog # A00292) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1/MAP2K1 at approximately 45 kDa. The expected band size for MEK1/MAP2K1 is at 45 kDa.
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