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ProductsBack/MEK7 (phospho Ser271/Thr275) antibody
WB analysis of background extracts with GST-tagged fusion protein expressing MEK7 using GTX24762 MEK7 (phospho Ser271/Thr275) antibody.
WB analysis of background extracts with GST-tagged fusion protein expressing MEK7 using GTX24762 MEK7 (phospho Ser271/Thr275) antibody.
WB analysis of background extracts with GST-tagged fusion protein expressing MEK7 using GTX24762 MEK7 (phospho Ser271/Thr275) antibody.

MEK7 (phospho Ser271/Thr275) antibody

GTX24762
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetMAP2K7
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Overview

  • Supplier
    GeneTex
  • Product Name
    MEK7 (phospho Ser271/Thr275) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-1 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5609
  • Target name
    MAP2K7
  • Target description
    mitogen-activated protein kinase kinase 7
  • Target synonyms
    c-Jun N-terminal kinase kinase 2; dual specificity mitogen-activated protein kinase kinase 7; JNK-activating kinase 2; JNKK2; MAP kinase kinase 7; MAPK/ERK kinase 7; MAPKK7; MEK; MEK 7; MKK7; PRKMK7; SAPK kinase 4; SAPKK4; SAPKK-4; stress-activated protein kinase kinase 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14733
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 7
  • Scientific Description
    The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MEK7/MAP2K7 using anti-MEK7/MAP2K7 antibody (PB9764). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat testis tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK7/MAP2K7 antigen affinity purified polyclonal antibody (Catalog # PB9764) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK7/MAP2K7 at approximately 47 kDa. The expected band size for MEK7/MAP2K7 is at 47 kDa.
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ELISA analysis of antigen using GTX60522 MEK7 antibody [4E5]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
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