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ProductsBack/Melanoma Marker (MART-1 + Tyrosinase + gp100)(DT101+ BC199 + T311 + HMB45), CF405S conjugate, 0.1mg/mL [26628-22-8]

Melanoma Marker (MART-1 + Tyrosinase + gp100)(DT101+ BC199 + T311 + HMB45), CF405S conjugate, 0.1mg/mL [26628-22-8]

BNC040704
Biotium
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse
TargetMLANA
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Overview

  • Supplier
    Biotium
  • Product Name
    Melanoma Marker (MART-1 + Tyrosinase + gp100)(DT101+ BC199 + T311 + HMB45), CF405S conjugate, 0.1mg/mL [26628-22-8]
  • Delivery Days Customer
    9
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DT101 BC199 T311 HMB45
  • Concentration
    0.1 mg/ml
  • Conjugate
    Other Conjugate
  • Gene ID2315
  • Target name
    MLANA
  • Target description
    melan-A
  • Target synonyms
    MART-1, MART1, melanoma antigen recognized by T-cells 1, antigen LB39-AA, antigen SK29-AA, epididymis secretory sperm binding protein, protein Melan-A
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
  • Source
    Animal
  • Reactivity
    Bovine, Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Melan-A/MLANA using anti-Melan-A/MLANA antibody (A02033-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Melan-A/MLANA antigen affinity purified polyclonal antibody (Catalog # A02033-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Melan-A/MLANA at approximately 13 kDa. The expected band size for Melan-A/MLANA is at 13 kDa.
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