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MER / MERTK Antibody

LS-C814054
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMERTK
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MER / MERTK Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10461
  • Target name
    MERTK
  • Target description
    MER proto-oncogene, tyrosine kinase
  • Target synonyms
    MER, RP38, Tyro12, c-Eyk, c-mer, tyrosine-protein kinase Mer, MER receptor tyrosine kinase, STK kinase, c-mer proto-oncogene tyrosine kinase, proto-oncogene c-Mer, receptor tyrosine kinase MerTK
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of MERTK using anti-MERTK antibody (A00489-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat lung tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MERTK antigen affinity purified polyclonal antibody (Catalog # A00489-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MERTK at approximately 120 kDa. The expected band size for MERTK is at 120 kDa.
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